Chromosomal imbalances in malignant peripheral nerve sheath tumor detected by metaphase and microarray comparative genomic hybridization

Yasuko Nakagawa, Aki Yoshida, Kunihiko Numoto, Toshiyuki Kunisada, Daniel Wai, Norihide Ohata, Ken Takeda, Akira Kawai, Toshihumi Ozaki

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Malignant peripheral nerve sheath tumors (MPNSTs) are highly malignant tumors affecting adolescents and adults. There have been a few reports on chromosomal aberrations of MPNSTs; however, the tumor-specific alteration remains unknown. We characterized the genomic alterations in 8 MPNSTs and 8 schwannomas by metaphase comparative genomic hybridization (CGH). In 5 of 8 MPNSTs, microarray CGH was added for more detailed analyses. Frequent gains were identified on 3q13-26, 5p13-14, and 12q11-23 and frequent losses were at 1p31, 10p, 11q24-qter, 16, and 17. Microarray CGH revealed frequent gains of EGFR, DAB2, MSH2, KCNK12, DDX15, CDK6, and LAMA3, and losses of CDH1, GLTSCR2, EGR1, CTSB, GATA3, and SULT2A1. These genes seem to be responsible for developing MPNSTs. The concordance rate between metaphase CGH and microarray CGH was 66%. Metaphase CGH was useful for identifying chromosomal alterations before applying microarray CGH.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalOncology Reports
Volume15
Issue number2
Publication statusPublished - Feb 2006

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Comparative Genomic Hybridization
Neurilemmoma
Metaphase
Chromosome Aberrations
Neoplasms
Genes

Keywords

  • Chromosomal imbalances
  • Genomic hybridization
  • Microarray
  • Nerve sheath tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Chromosomal imbalances in malignant peripheral nerve sheath tumor detected by metaphase and microarray comparative genomic hybridization. / Nakagawa, Yasuko; Yoshida, Aki; Numoto, Kunihiko; Kunisada, Toshiyuki; Wai, Daniel; Ohata, Norihide; Takeda, Ken; Kawai, Akira; Ozaki, Toshihumi.

In: Oncology Reports, Vol. 15, No. 2, 02.2006, p. 297-303.

Research output: Contribution to journalArticle

Nakagawa, Yasuko ; Yoshida, Aki ; Numoto, Kunihiko ; Kunisada, Toshiyuki ; Wai, Daniel ; Ohata, Norihide ; Takeda, Ken ; Kawai, Akira ; Ozaki, Toshihumi. / Chromosomal imbalances in malignant peripheral nerve sheath tumor detected by metaphase and microarray comparative genomic hybridization. In: Oncology Reports. 2006 ; Vol. 15, No. 2. pp. 297-303.
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