Chromosomal assignment and deletion mapping of barley EST markers

Shuhei Nasuda, Yukari Kikkawa, Taizo Ashida, A. K.M.Rafiqul Islam, Kazuhiro Sato, Takashi R. Endo

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.

Original languageEnglish
Pages (from-to)357-366
Number of pages10
JournalGenes and Genetic Systems
Volume80
Issue number5
DOIs
Publication statusPublished - Dec 1 2005

Keywords

  • Barley
  • Chromosome mapping
  • EST
  • Wheat-barley addition

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Nasuda, S., Kikkawa, Y., Ashida, T., Islam, A. K. M. R., Sato, K., & Endo, T. R. (2005). Chromosomal assignment and deletion mapping of barley EST markers. Genes and Genetic Systems, 80(5), 357-366. https://doi.org/10.1266/ggs.80.357