Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping

Toshifumi Ozaki, Thomas Neumann, Daniel Wai, Karl Ludwig Schäfer, Franz Van Valen, Norbert Lindner, Christina Scheel, Werner Böcker, Winfried Winkelmann, Barbara Dockhorn-Dworniczak, Jürgen Horst, Christopher Poremba

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31 Citations (Scopus)

Abstract

We characterized the chromosomal alterations in eight osteosarcoma cell lines (OST, HOS, U-2 OS, ZK-58, MG-63, SJSA-1, Saos-2, and MNNG) by comparative genomic hybridization (CGH); gains and losses of DNA sequences were defined as chromosomal regions with a fluorescence ratio, wherein all of the 95% confidence interval was above 1.25 and below 0.75, respectively. In four of 8 cell lines, multicolor karyotyping (MK) was added. CGH revealed the average number of aberrations per cell line was 20.8 (range: 10-31); the average numbers of gains and losses were 11.1 and 9.6, respectively. The frequent gains were identified on 1p21∼q24, 1q25∼q31, 7p21, 7q31, 8q23∼q24, and 14q21; frequent losses were at 18q21∼q22, 18q12, 19p, and 3p12∼p14. High-level gains were observed on 8q23∼q24, 5p, and 1p21∼p22. MK revealed the most common translocations in the four cell lines were t(8;9), t(1;3), t(3;5), t(1;13), t(2;6), t(3;17), t(1;15), t(10;20), and t(6;20). Chromosomes 1, 3, 8, 9, and 20 were most frequently involved in translocation events. The concordance rate of aberrations in CGH and translocations in MK was 76%. MK was useful to identify the chromosomal alterations and as a supplement to the CGH results in three of four chromosomes.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalCancer Genetics and Cytogenetics
Volume140
Issue number2
DOIs
Publication statusPublished - Jan 15 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Ozaki, T., Neumann, T., Wai, D., Schäfer, K. L., Van Valen, F., Lindner, N., Scheel, C., Böcker, W., Winkelmann, W., Dockhorn-Dworniczak, B., Horst, J., & Poremba, C. (2003). Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping. Cancer Genetics and Cytogenetics, 140(2), 145-152. https://doi.org/10.1016/S0165-4608(02)00685-4