Chromatography of tannins. IV. Separation of labile oligomeric hydrolysable tannins and related polyphenols by centrifugal partition chromatography

Takashi Yoshida, Tsutomu Hatano, Takuo Okuda

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Using centrifugal partition chromatography for the separation and purification of oligomeric hydrolysable tannins extracted from Heterocentron roseum, a labile trimer, nobotanin J, which was readily decomposed into a monomer and dimer(s) on a gel column chromatographic solid support, and a more polar tetramer, nobotanin K, were efficiently purified by normal- and reversed-phase developments using the solvent system n-butanol-n-propanol-water (4:1:5, v/v/v). Licochalcone B, a polyphenol having tannin-like activity, was also separated from other components with related structures in Sinkiang licorice extract by centrifugal partition chromatography with normal-phase development using chloroform-methanol-water (7:13:8, v/v/v) as the solvent system.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 1989


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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