Chromatography of tannins. II. Preparative fractionation of hydrolyzable tannins by centrifugal partition chromatography

Centrifugal partition chromatography with an apparatus which employs rotary joints has been applied to the separation of tannins in extracts from several plant species. The results were compared with those obtainable by droplet counter-current chromatography. Combined applications of normal- and reversed-phase developments, and of centrifugal partition chromatography and other chromatographic methods, were also carried out. The results show that centrifugal partition chromatography shortens the development time to a quarter of that with droplet counter-current chromatography, and enables separation of some tannins whose isolation by droplet counter-current chromatography is impracticable. It is also demonstrated that centrifugal partition chromatography is a suitable method, particularly on the preparative scale, for the separation of tannins.