Abstract
The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
| Original language | English |
|---|---|
| Pages (from-to) | 1221-1225 |
| Number of pages | 5 |
| Journal | Genetics |
| Volume | 163 |
| Issue number | 3 |
| Publication status | Published - Mar 1 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. / Nagaki, Kiyotaka; Talbert, Paul B.; Zhong, Cathy Xiaoyan; Dawe, R. Kelly; Henikoff, Steven; Jiang, Jiming.
In: Genetics, Vol. 163, No. 3, 01.03.2003, p. 1221-1225.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres
AU - Nagaki, Kiyotaka
AU - Talbert, Paul B.
AU - Zhong, Cathy Xiaoyan
AU - Dawe, R. Kelly
AU - Henikoff, Steven
AU - Jiang, Jiming
PY - 2003/3/1
Y1 - 2003/3/1
N2 - The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
AB - The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
UR - http://www.scopus.com/inward/record.url?scp=0037355216&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037355216&partnerID=8YFLogxK
M3 - Article
VL - 163
SP - 1221
EP - 1225
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 3
ER -