Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki, Paul B. Talbert, Cathy Xiaoyan Zhong, R. Kelly Dawe, Steven Henikoff, Jiming Jiang

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

Original languageEnglish
Pages (from-to)1221-1225
Number of pages5
JournalGenetics
Volume163
Issue number3
Publication statusPublished - Mar 1 2003
Externally publishedYes

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Centromere
Chromatin Immunoprecipitation
Arabidopsis
DNA
Kinetochores
Antibodies
Histones
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. / Nagaki, Kiyotaka; Talbert, Paul B.; Zhong, Cathy Xiaoyan; Dawe, R. Kelly; Henikoff, Steven; Jiang, Jiming.

In: Genetics, Vol. 163, No. 3, 01.03.2003, p. 1221-1225.

Research output: Contribution to journalArticle

Nagaki, Kiyotaka ; Talbert, Paul B. ; Zhong, Cathy Xiaoyan ; Dawe, R. Kelly ; Henikoff, Steven ; Jiang, Jiming. / Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. In: Genetics. 2003 ; Vol. 163, No. 3. pp. 1221-1225.
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