Abstract
The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
| Original language | English |
|---|---|
| Pages (from-to) | 1221-1225 |
| Number of pages | 5 |
| Journal | Genetics |
| Volume | 163 |
| Issue number | 3 |
| Publication status | Published - Mar 1 2003 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics
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Cite this
Nagaki, K., Talbert, P. B., Zhong, C. X., Dawe, R. K., Henikoff, S., & Jiang, J. (2003). Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. Genetics, 163(3), 1221-1225.