Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki, Paul B. Talbert, Cathy Xiaoyan Zhong, R. Kelly Dawe, Steven Henikoff, Jiming Jiang

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

Original languageEnglish
Pages (from-to)1221-1225
Number of pages5
JournalGenetics
Volume163
Issue number3
Publication statusPublished - Mar 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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