Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki; Paul B. Talbert; Cathy Xiaoyan Zhong; R. Kelly Dawe; Steven Henikoff; Jiming Jiang

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki, Paul B. Talbert, Cathy Xiaoyan Zhong, R. Kelly Dawe, Steven Henikoff, Jiming Jiang

Research output: Contribution to journal › Article › peer-review

Abstract

The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

Original language	English
Pages (from-to)	1221-1225
Number of pages	5
Journal	Genetics
Volume	163
Issue number	3
Publication status	Published - Mar 1 2003
Externally published	Yes

ASJC Scopus subject areas

Genetics

Cite this

@article{8b6530464973477396f34457bbc3fa17,

title = "Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres",

abstract = "The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.",

author = "Kiyotaka Nagaki and Talbert, {Paul B.} and Zhong, {Cathy Xiaoyan} and Dawe, {R. Kelly} and Steven Henikoff and Jiming Jiang",

year = "2003",

month = mar,

day = "1",

language = "English",

volume = "163",

pages = "1221--1225",

journal = "Genetics",

issn = "0016-6731",

publisher = "Genetics Society of America",

number = "3",

}

TY - JOUR

T1 - Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

AU - Nagaki, Kiyotaka

AU - Talbert, Paul B.

AU - Zhong, Cathy Xiaoyan

AU - Dawe, R. Kelly

AU - Henikoff, Steven

AU - Jiang, Jiming

PY - 2003/3/1

Y1 - 2003/3/1

N2 - The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

AB - The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

UR - http://www.scopus.com/inward/record.url?scp=0037355216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037355216&partnerID=8YFLogxK

M3 - Article

C2 - 12663558

AN - SCOPUS:0037355216

VL - 163

SP - 1221

EP - 1225

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 3

ER -

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this