Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki; Paul B. Talbert; Cathy Xiaoyan Zhong; R. Kelly Dawe; Steven Henikoff; Jiming Jiang

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Kiyotaka Nagaki, Paul B. Talbert, Cathy Xiaoyan Zhong, R. Kelly Dawe, Steven Henikoff, Jiming Jiang

Research output: Contribution to journal › Article › peer-review

Abstract

The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

Original language	English
Pages (from-to)	1221-1225
Number of pages	5
Journal	Genetics
Volume	163
Issue number	3
Publication status	Published - Mar 1 2003
Externally published	Yes

ASJC Scopus subject areas

Genetics

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Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. / Nagaki, Kiyotaka; Talbert, Paul B.; Zhong, Cathy Xiaoyan; Dawe, R. Kelly; Henikoff, Steven; Jiang, Jiming.

In: Genetics, Vol. 163, No. 3, 01.03.2003, p. 1221-1225.

Research output: Contribution to journal › Article › peer-review

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AB - The centromeres ot Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

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Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres

Abstract

ASJC Scopus subject areas

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