Chromatin immunoprecipitation for detecting epigenetic marks on plant nucleosomes

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Abstract

Due to high resolution and reproducibility, chromatin immunoprecipitation (ChIP) has been used as a standard tool to investigate epigenetic marks including modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and lowbackground ChIP protocol for a wide range of plant species.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages197-206
Number of pages10
Volume1469
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1469
ISSN (Print)1064-3745

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Keywords

  • CENH3
  • Chromatin immunoprecipitation (CHIP)
  • Epigenetic marks
  • Histone modification
  • Histone variants

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Nagaki, K. (2016). Chromatin immunoprecipitation for detecting epigenetic marks on plant nucleosomes. In Methods in Molecular Biology (Vol. 1469, pp. 197-206). (Methods in Molecular Biology; Vol. 1469). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4931-1_16