Chromaffin granule H+-ATPase has F1-like structure

Yoshinori Moriyama, Akitsugu Yamamoto, Yutaka Tashiro, Masamitsu Futai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Isolated H+-ATPase from chromaffin granules was reconstituted into liposomes and the resultant proteoliposomes were further purified by Ficoll density gradient centrifugation. Studies by electron microscopy showed that proteoliposomes had particle structures (average diameter, about 10 nm) on their outer surface. These particles could be removed from the proteoliposomes by cold treatment. Immuno-electron microscopy showed that these particles were recognized by antibodies against the hydrophilic sector of the enzyme. These results indicate that the H+-ATPase has a peripheral membrane structure similar to that of F1-ATPase.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalFEBS Letters
Volume291
Issue number1
DOIs
Publication statusPublished - Oct 7 1991
Externally publishedYes

Fingerprint

Chromaffin Granules
Proton-Translocating ATPases
Electron microscopy
Ficoll
Membrane structures
Immunoelectron Microscopy
Density Gradient Centrifugation
Centrifugation
Liposomes
Electron Microscopy
Membranes
Antibodies
Enzymes
proteoliposomes
Therapeutics

Keywords

  • Catalytic sector
  • Chromaffin granule
  • Extrinsic membrane sector
  • F-like particle
  • Vacuolar H-ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Moriyama, Y., Yamamoto, A., Tashiro, Y., & Futai, M. (1991). Chromaffin granule H+-ATPase has F1-like structure. FEBS Letters, 291(1), 92-96. https://doi.org/10.1016/0014-5793(91)81111-K

Chromaffin granule H+-ATPase has F1-like structure. / Moriyama, Yoshinori; Yamamoto, Akitsugu; Tashiro, Yutaka; Futai, Masamitsu.

In: FEBS Letters, Vol. 291, No. 1, 07.10.1991, p. 92-96.

Research output: Contribution to journalArticle

Moriyama, Y, Yamamoto, A, Tashiro, Y & Futai, M 1991, 'Chromaffin granule H+-ATPase has F1-like structure', FEBS Letters, vol. 291, no. 1, pp. 92-96. https://doi.org/10.1016/0014-5793(91)81111-K
Moriyama Y, Yamamoto A, Tashiro Y, Futai M. Chromaffin granule H+-ATPase has F1-like structure. FEBS Letters. 1991 Oct 7;291(1):92-96. https://doi.org/10.1016/0014-5793(91)81111-K
Moriyama, Yoshinori ; Yamamoto, Akitsugu ; Tashiro, Yutaka ; Futai, Masamitsu. / Chromaffin granule H+-ATPase has F1-like structure. In: FEBS Letters. 1991 ; Vol. 291, No. 1. pp. 92-96.
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