Chromaffin cell survival from both young and old donors is enhanced by co-grafts of polymer-encapsulated human NGF-secreting cells

Isao Date, Takashi Ohmoto, Takashi Imaoka, Tetsuro Shingo, Dwaine F. Emerich

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Following polymer-encapsulation, human nerve growth factor-secreting baby hamster kidney fibroblasts (BHK-hNGF) were implanted into the striatum of hemiparkinsonian rats together with unencapsulated adrenal medullary chromaffin cells from either young (2 weeks) or old (12 months) donor rats. Animals receiving both BHK-hNGF cells and chromaffin cells exhibited significant decreases (39-56%) in apomorphine-induced rotational behaviour which was equivalent regardless of the age of the donor tissue. Histological analysis revealed that while survival of chromaffin cells without hNGF support was poor, co-grafts of adrenal medulla and BHK/hNGF cells increased chromaffin cell survival by 20 times. Again, this effect was independent of the age of the donor tissue. Retrieved capsules contained numerous viable encapsulated BHK-hNGF cells which continued to release hNGF. These results further indicate the potential use of intrastriatal implantation of encapsulated hNGF-secreting cells for augmenting the survival of co-grafted chromaffin cells as well as promoting the functional recovery of hemiparkinsonian rats.

Original languageEnglish
Pages (from-to)1813-1818
Number of pages6
JournalNeuroReport
Volume7
Issue number11
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • chromaffin cell
  • fibroblast
  • nerve growth factor
  • neural transplantation
  • polymer

ASJC Scopus subject areas

  • Neuroscience(all)

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