Abstract
The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.
| Original language | English |
|---|---|
| Pages (from-to) | 535-543 |
| Number of pages | 9 |
| Journal | Archives of Histology and Cytology |
| Volume | 64 |
| Issue number | 5 |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Anatomy
- Histology
Cite this
Chondroitin sulfate proteoglycan at the basal lamina beneath high endothelial cells in human palatine tonsils : A light and electron microscopic study using the cationic colloidal iron method. / Kataoka, Yuko; Akagi, Hirofumi; Nishizaki, Kazunori; Taguchi, Takehito; Murakami, Takuro; Ohtsuka, Aiji.
In: Archives of Histology and Cytology, Vol. 64, No. 5, 2001, p. 535-543.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chondroitin sulfate proteoglycan at the basal lamina beneath high endothelial cells in human palatine tonsils
T2 - A light and electron microscopic study using the cationic colloidal iron method
AU - Kataoka, Yuko
AU - Akagi, Hirofumi
AU - Nishizaki, Kazunori
AU - Taguchi, Takehito
AU - Murakami, Takuro
AU - Ohtsuka, Aiji
PY - 2001
Y1 - 2001
N2 - The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.
AB - The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035705405&partnerID=8YFLogxK
M3 - Article
C2 - 11838713
AN - SCOPUS:0035705405
VL - 64
SP - 535
EP - 543
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 5
ER -