Chondroitin sulfate proteoglycan at the basal lamina beneath high endothelial cells in human palatine tonsils: A light and electron microscopic study using the cationic colloidal iron method

Yuko Kataoka, Hirofumi Akagi, Kazunori Nishizaki, Takehito Taguchi, Takuro Murakami, Aiji Ohtsuka

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Abstract

The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.

Original languageEnglish
Pages (from-to)535-543
Number of pages9
JournalArchives of Histology and Cytology
Volume64
Issue number5
Publication statusPublished - 2001

Fingerprint

Chondroitin Sulfate Proteoglycans
Palatine Tonsil
Venules
Basement Membrane
Rosaniline Dyes
Iron
Endothelial Cells
Electrons
Light
Aldehydes
Staining and Labeling
Digestion
keratan-sulfate endo-1,4-beta-galactosidase
Neuraminidase
heparitinsulfate lyase
Lymphocytes
Chondroitin ABC Lyase
Hyaluronoglucosaminidase
Chondroitin Sulfates
Collagenases

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

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abstract = "The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.",
author = "Yuko Kataoka and Hirofumi Akagi and Kazunori Nishizaki and Takehito Taguchi and Takuro Murakami and Aiji Ohtsuka",
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T1 - Chondroitin sulfate proteoglycan at the basal lamina beneath high endothelial cells in human palatine tonsils

T2 - A light and electron microscopic study using the cationic colloidal iron method

AU - Kataoka, Yuko

AU - Akagi, Hirofumi

AU - Nishizaki, Kazunori

AU - Taguchi, Takehito

AU - Murakami, Takuro

AU - Ohtsuka, Aiji

PY - 2001

Y1 - 2001

N2 - The basal lamina of high endothelial venules (HEVs) in human palatine tonsils was intensely stained with cationic colloidal iron at pH 1.5 and with aldehyde fuchsin. This basal lamina exhibited a thick and doubleor triple-layered structure forming small compartments, in which many lymphocytes were aligned. Digestion with hyaluronidase or collagenase eliminated both the colloidal iron and aldehyde fuchsin stainings of the basal lamina of HEVs. Treatment with chondroitinase ABC reduced colloidal iron staining, but did not interfere with the aldehyde fuchsin staining. Digestion with neuraminidase, keratanase, or heparitinase did not eliminate either the cationic colloidal or the aldehyde fuchsin staining. Digestion with neuraminidase reduced the colloidal iron staining on the luminal surface coat of the HEV. Electron microscopy of ultrathin sections revealed that cationic colloidal iron particles were deposited on the basal lamina of the HEV. The basal laminae of ordinary blood vessels were thin and singlelayered, and stained only weakly with cationic colloidal iron. The present study suggests that negatively charged sites in the basal lamina of HEV derive mainly from a proteoglycan complex containing hyaluronic acid and chondroitin sulfate, which firmly binds collagen. This topochemical feature is suggested to be involved in the fascilitating migration of lymphocytes after passage through the endothelial layer.

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