Cholesteryl ester transfer protein expressed in lecithin cholesterol acyltransferase-deficient mice

Cheng Ai Wu, Maki Tsujita, Kuniko Okumura-Noji, Shinichi Usui, Hajime Kakuuchi, Mitsuyo Okazaki, Shinji Yokoyama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective - Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. Methods and Results - LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/-) mice, plasma CETP varied from 0 to 30 μg/mL, whereas it was <10 μg/mL in the lcat(-/-) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/-) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the Icat(-/-) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat(-/-) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat(-/-) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat(-/-) mouse. Conclusions - Plasma CETP is posttranscriptionally downregulated in the lcat(-/-) mice, presumably by its extremely low HDL.

Original languageEnglish
Pages (from-to)1347-1353
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number8
DOIs
Publication statusPublished - Aug 23 2002
Externally publishedYes

Keywords

  • Cholesterol
  • Cholesteryl ester transfer protein
  • High density lipoprotein
  • Lecithin-cholesterol acyltransferase
  • Mice

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Cholesteryl ester transfer protein expressed in lecithin cholesterol acyltransferase-deficient mice'. Together they form a unique fingerprint.

  • Cite this