Cholecystokinin plays a novel protective role in diabetic kidney through anti-inflammatory actions on macrophage

Anti-inflammatory effect of cholecystokinin

Satoshi Miyamoto, Kenichi Shikata, Kyoko Miyasaka, Shinichi Okada, Motofumi Sasaki, Ryo Kodera, Daisho Hirota, Nobuo Kajitani, Tetsuharu Takatsuka, Hitomi Kataoka, Shingo Nishishita, Chikage Sato, Akihiro Funakoshi, Hisakazu Nishimori, Haruhito Adam Uchida, Daisuke Ogawa, Hirofumi Makino

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Inflammatory process is involved in the pathogenesis of diabetic nephropathy. In this article, we show that cholecystokinin (CCK) is expressed in the kidney and exerts renoprotective effects through its anti-inflammatory actions. DNA microarray showed that CCK was upregulated in the kidney of diabetic wild-type (WT) mice but not in diabetic intracellular adhesion molecule-1 knockout mice. We induced diabetes in CCK-1 receptor (CCK-1R) and CCK-2R double-knockout (CCK-1R -/-,-2R -/-) mice, and furthermore, we performed a bone marrow transplantation study using CCK-1R -/- mice to determine the role of CCK-1R on macrophages in the diabetic kidney. Diabetic CCK-1R -/-,-2R -/-mice revealed enhanced albuminuria and inflammation in the kidney compared with diabetic WT mice. In addition, diabetic WT mice with CCK-1R -/- bone marrow-derived cells developed more albuminuria than diabetic CCK-1R -/- mice with WT bone marrow-derived cells. Administration of sulfated cholecystokinin octapeptide (CCK-8S) ameliorated albuminuria, podocyte loss, expression of proinflammatory genes, and infiltration of macrophages in the kidneys of diabetic rats. Furthermore, CCK-8S inhibited both expression of tumor necrosis factor-a and chemotaxis in cultured THP-1 cells. These results suggest that CCK suppresses the activation of macrophage and expression of proinflammatory genes in diabetic kidney. Our findings may provide a novel strategy of therapy for the early stage of diabetic nephropathy.

Original languageEnglish
Pages (from-to)897-907
Number of pages11
JournalDiabetes
Volume61
Issue number4
DOIs
Publication statusPublished - Apr 2012

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Cholecystokinin
Anti-Inflammatory Agents
Macrophages
Kidney
Albuminuria
Diabetic Nephropathies
Knockout Mice
Bone Marrow Cells
Cholecystokinin Receptors
Gene Expression
Sincalide
Podocytes
Macrophage Activation
Chemotaxis
Oligonucleotide Array Sequence Analysis
Bone Marrow Transplantation
Tumor Necrosis Factor-alpha
Inflammation

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Cholecystokinin plays a novel protective role in diabetic kidney through anti-inflammatory actions on macrophage : Anti-inflammatory effect of cholecystokinin. / Miyamoto, Satoshi; Shikata, Kenichi; Miyasaka, Kyoko; Okada, Shinichi; Sasaki, Motofumi; Kodera, Ryo; Hirota, Daisho; Kajitani, Nobuo; Takatsuka, Tetsuharu; Kataoka, Hitomi; Nishishita, Shingo; Sato, Chikage; Funakoshi, Akihiro; Nishimori, Hisakazu; Uchida, Haruhito Adam; Ogawa, Daisuke; Makino, Hirofumi.

In: Diabetes, Vol. 61, No. 4, 04.2012, p. 897-907.

Research output: Contribution to journalArticle

Miyamoto, Satoshi ; Shikata, Kenichi ; Miyasaka, Kyoko ; Okada, Shinichi ; Sasaki, Motofumi ; Kodera, Ryo ; Hirota, Daisho ; Kajitani, Nobuo ; Takatsuka, Tetsuharu ; Kataoka, Hitomi ; Nishishita, Shingo ; Sato, Chikage ; Funakoshi, Akihiro ; Nishimori, Hisakazu ; Uchida, Haruhito Adam ; Ogawa, Daisuke ; Makino, Hirofumi. / Cholecystokinin plays a novel protective role in diabetic kidney through anti-inflammatory actions on macrophage : Anti-inflammatory effect of cholecystokinin. In: Diabetes. 2012 ; Vol. 61, No. 4. pp. 897-907.
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abstract = "Inflammatory process is involved in the pathogenesis of diabetic nephropathy. In this article, we show that cholecystokinin (CCK) is expressed in the kidney and exerts renoprotective effects through its anti-inflammatory actions. DNA microarray showed that CCK was upregulated in the kidney of diabetic wild-type (WT) mice but not in diabetic intracellular adhesion molecule-1 knockout mice. We induced diabetes in CCK-1 receptor (CCK-1R) and CCK-2R double-knockout (CCK-1R -/-,-2R -/-) mice, and furthermore, we performed a bone marrow transplantation study using CCK-1R -/- mice to determine the role of CCK-1R on macrophages in the diabetic kidney. Diabetic CCK-1R -/-,-2R -/-mice revealed enhanced albuminuria and inflammation in the kidney compared with diabetic WT mice. In addition, diabetic WT mice with CCK-1R -/- bone marrow-derived cells developed more albuminuria than diabetic CCK-1R -/- mice with WT bone marrow-derived cells. Administration of sulfated cholecystokinin octapeptide (CCK-8S) ameliorated albuminuria, podocyte loss, expression of proinflammatory genes, and infiltration of macrophages in the kidneys of diabetic rats. Furthermore, CCK-8S inhibited both expression of tumor necrosis factor-a and chemotaxis in cultured THP-1 cells. These results suggest that CCK suppresses the activation of macrophage and expression of proinflammatory genes in diabetic kidney. Our findings may provide a novel strategy of therapy for the early stage of diabetic nephropathy.",
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