Chloroplast-encoded PsbT is required for efficient biogenesis of photosystem II complex in the green alga Chlamydomonas reinhardtii

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The small hydrophobic polypeptide PsbT is associated with the photosystem II (PSII) reaction center (D1/D2 heterodimer). Here, we report the effect of the deletion of PsbT on the biogenesis of PSII complex during light-induced greening of y-1 mutants of the green alga Chlamydomonas reinhardtii. The y-1 is unable to synthesize chlorophylls in the dark but do so in the light. The dark-grown y-1 cells accumulated no major PSII proteins but a small amount of PsbT. Upon illumination, PsbT was immediately synthesized while chlorophylls, major PSII proteins, and O2-evolving activity increased after a 1-h lag. The y-1 cells without PsbT accumulated chlorophylls and PSI protein at a similar rate, whereas the accumulation of PSII complex was specifically retarded during greening. The absence of PsbT did not affect the synthesis of PSII proteins. These results indicate that PsbT is required for the efficient biogenesis of PSII complex.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalPhotosynthesis research
Volume98
Issue number1-3
DOIs
Publication statusPublished - Oct 1 2008

Keywords

  • Assembly
  • Chlorophyll-protein complex
  • Greening
  • Photosynthesis
  • Pulse-labeling
  • Reaction center
  • y-1 mutant

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Chloroplast-encoded PsbT is required for efficient biogenesis of photosystem II complex in the green alga Chlamydomonas reinhardtii'. Together they form a unique fingerprint.

Cite this