Abstract
De-etiolation of dark-grown seedlings is a commonly used experimental system to study the mechanisms of chloroplast biogenesis, including the stacking of thylakoid membranes into grana, the response of the nuclear-chloroplast transcriptome to light, and the ordered synthesis and assembly of photosystem II (PSII). Here, we present the xeroplast to chloroplast transition during rehydration of the resurrection plant Xerophyta humilis as a novel system for studying chloroplast biogenesis, and investigate the role of light in this process. Xeroplasts are characterized by the presence of numerous large and small membrane-bound vesicles and the complete absence of thylakoid membranes. While the initial assembly of stromal thylakoid membranes occurs independently of light, the formation of grana is light dependent. Recovery of photosynthetic activity is rapid in plants rehydrated in the light and correlates with the light-dependent synthesis of the D1 protein, but does not require de novo chlorophyll biosynthesis. Light-dependent synthesis of the chlorophyll-binding protein Lhcb2 and digalactosyldiacylglycerol synthase 1 correlated with the formation of grana and with the increased PSII activity. Our results suggest that the molecular mechanisms underlying photomorphogenic development may also function in desiccation tolerance in poikilochlorophyllous resurrection plants.
| Original language | English |
|---|---|
| Pages (from-to) | 1813-1824 |
| Number of pages | 12 |
| Journal | Plant, Cell and Environment |
| Volume | 31 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2008 |
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Keywords
- Desiccation tolerance
- Photosynthesis
- Resurrection plant
- Xeroplast
ASJC Scopus subject areas
- Plant Science
- Physiology
Cite this
Chloroplast biogenesis during rehydration of the resurrection plant Xerophyta humilis : Parallels to the etioplast-chloroplast transition. / Ingle, Robert A.; Collett, Helen; Cooper, Keren; Takahashi, Yuichiro; Farrant, Jill M.; Illing, Nicola.
In: Plant, Cell and Environment, Vol. 31, No. 12, 12.2008, p. 1813-1824.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chloroplast biogenesis during rehydration of the resurrection plant Xerophyta humilis
T2 - Parallels to the etioplast-chloroplast transition
AU - Ingle, Robert A.
AU - Collett, Helen
AU - Cooper, Keren
AU - Takahashi, Yuichiro
AU - Farrant, Jill M.
AU - Illing, Nicola
PY - 2008/12
Y1 - 2008/12
N2 - De-etiolation of dark-grown seedlings is a commonly used experimental system to study the mechanisms of chloroplast biogenesis, including the stacking of thylakoid membranes into grana, the response of the nuclear-chloroplast transcriptome to light, and the ordered synthesis and assembly of photosystem II (PSII). Here, we present the xeroplast to chloroplast transition during rehydration of the resurrection plant Xerophyta humilis as a novel system for studying chloroplast biogenesis, and investigate the role of light in this process. Xeroplasts are characterized by the presence of numerous large and small membrane-bound vesicles and the complete absence of thylakoid membranes. While the initial assembly of stromal thylakoid membranes occurs independently of light, the formation of grana is light dependent. Recovery of photosynthetic activity is rapid in plants rehydrated in the light and correlates with the light-dependent synthesis of the D1 protein, but does not require de novo chlorophyll biosynthesis. Light-dependent synthesis of the chlorophyll-binding protein Lhcb2 and digalactosyldiacylglycerol synthase 1 correlated with the formation of grana and with the increased PSII activity. Our results suggest that the molecular mechanisms underlying photomorphogenic development may also function in desiccation tolerance in poikilochlorophyllous resurrection plants.
AB - De-etiolation of dark-grown seedlings is a commonly used experimental system to study the mechanisms of chloroplast biogenesis, including the stacking of thylakoid membranes into grana, the response of the nuclear-chloroplast transcriptome to light, and the ordered synthesis and assembly of photosystem II (PSII). Here, we present the xeroplast to chloroplast transition during rehydration of the resurrection plant Xerophyta humilis as a novel system for studying chloroplast biogenesis, and investigate the role of light in this process. Xeroplasts are characterized by the presence of numerous large and small membrane-bound vesicles and the complete absence of thylakoid membranes. While the initial assembly of stromal thylakoid membranes occurs independently of light, the formation of grana is light dependent. Recovery of photosynthetic activity is rapid in plants rehydrated in the light and correlates with the light-dependent synthesis of the D1 protein, but does not require de novo chlorophyll biosynthesis. Light-dependent synthesis of the chlorophyll-binding protein Lhcb2 and digalactosyldiacylglycerol synthase 1 correlated with the formation of grana and with the increased PSII activity. Our results suggest that the molecular mechanisms underlying photomorphogenic development may also function in desiccation tolerance in poikilochlorophyllous resurrection plants.
KW - Desiccation tolerance
KW - Photosynthesis
KW - Resurrection plant
KW - Xeroplast
UR - http://www.scopus.com/inward/record.url?scp=55949124473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55949124473&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3040.2008.01887.x
DO - 10.1111/j.1365-3040.2008.01887.x
M3 - Article
C2 - 18771571
AN - SCOPUS:55949124473
VL - 31
SP - 1813
EP - 1824
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 12
ER -