Chitosan signaling in guard cells requires endogenous salicylic acid

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5 Citations (Scopus)

Abstract

An elicitor chitosan (CHT) induces stomatal closure but the mechanism remains to be clarified. A phytohormone salicylic acid (SA) is crucial for elicitor-induced defense signaling in plants. Here we investigated whether endogenous SA is required for CHT signaling in guard cells. In the SA-deficient nahG mutant, treatment of CHT did not induce either apoplastic reactive oxygen species (ROS) production or stomatal closure but co-treatment of CHT and SA induced both apoplastic ROS production and stomatal closure, indicating the involvement of endogenous SA in CHT-induced apoplastic ROS production and CHT-induced stomatal closure. Furthermore, CHT induced transient cytosolic free calcium concentration increments in the nahG mutant in the presence of exogenous SA but not in the absence of exogenous SA. These results provide evidence that endogenous SA is a crucial element in CHT-induced stomatal closure.

Original languageEnglish
Pages (from-to)1536-1541
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume81
Issue number8
DOIs
Publication statusPublished - 2017

Keywords

  • Chitosan
  • Cytosolic Ca oscillations
  • Reactive oxygen species
  • Salicylic acid
  • Stomatal closure

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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