Abstract
Vibrio cholerae regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in V. cholerae is an interbacterial killing device. This system is thought to provide a competitive advantage to V. cholerae in a polymicrobial community of the aquatic region under nutrient-poor conditions. V. cholerae chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc2 (N,N′- diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in V. cholerae is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in V. cholerae. ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that V. cholerae colonization on chitinous surfaces activates ChiS, which promotes T6SSdependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation.
| Original language | English |
|---|---|
| Article number | 000656 |
| Pages (from-to) | 751-763 |
| Number of pages | 13 |
| Journal | Microbiology (United Kingdom) |
| Volume | 164 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2018 |
| Externally published | Yes |
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Keywords
- ChiS activation
- Chitin
- Chitinases
- T6SS
- Vibrio cholerae
ASJC Scopus subject areas
- Microbiology
Cite this
Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation. / Chourashi, Rhishita; Das, Suman; Dhar, Debarpan; Okamoto, Keinosuke; Mukhopadhyay, Asish K.; Chatterjee, Nabendu Sekhar.
In: Microbiology (United Kingdom), Vol. 164, No. 5, 000656, 01.05.2018, p. 751-763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation
AU - Chourashi, Rhishita
AU - Das, Suman
AU - Dhar, Debarpan
AU - Okamoto, Keinosuke
AU - Mukhopadhyay, Asish K.
AU - Chatterjee, Nabendu Sekhar
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Vibrio cholerae regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in V. cholerae is an interbacterial killing device. This system is thought to provide a competitive advantage to V. cholerae in a polymicrobial community of the aquatic region under nutrient-poor conditions. V. cholerae chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc2 (N,N′- diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in V. cholerae is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in V. cholerae. ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that V. cholerae colonization on chitinous surfaces activates ChiS, which promotes T6SSdependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation.
AB - Vibrio cholerae regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in V. cholerae is an interbacterial killing device. This system is thought to provide a competitive advantage to V. cholerae in a polymicrobial community of the aquatic region under nutrient-poor conditions. V. cholerae chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc2 (N,N′- diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in V. cholerae is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in V. cholerae. ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that V. cholerae colonization on chitinous surfaces activates ChiS, which promotes T6SSdependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation.
KW - ChiS activation
KW - Chitin
KW - Chitinases
KW - T6SS
KW - Vibrio cholerae
UR - http://www.scopus.com/inward/record.url?scp=85046409471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046409471&partnerID=8YFLogxK
U2 - 10.1099/mic.0.000656
DO - 10.1099/mic.0.000656
M3 - Article
C2 - 29633936
AN - SCOPUS:85046409471
VL - 164
SP - 751
EP - 763
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 5
M1 - 000656
ER -