Chinese Spring Wheat (Triticum aestivum L.) Chloroplast Genome: Complete Sequence and Contig Clones

Yasunari Ogihara, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri, Koichiro Tsunewaki

    Research output: Contribution to journalArticlepeer-review

    52 Citations (Scopus)

    Abstract

    Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

    Original languageEnglish
    Pages (from-to)243-253
    Number of pages11
    JournalPlant Molecular Biology Reporter
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - 2000

    Keywords

    • Chinese Spring wheat
    • Chloroplast DNA
    • Clone bank
    • Complete nucleotide sequence
    • Plastome evolution

    ASJC Scopus subject areas

    • Molecular Biology
    • Plant Science

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