Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus

Kaori Terasaki, Haruko Hirayama, Christopher J. Kasanga, Min Thein Maw, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Susceptibility of DT40 cells to pathogenic field strains of infectious bursal disease virus (IBDV) including very virulent and classical virulent strains were studied. After the first and second passage of the virus in DT40 cells, IBDV-specific antigen was readily detected in DT40 cells inoculated with the pathogenic field strain infected bursal homogenates. Nucleotide sequence analysis in the VP2 hypervariable domain, which is critical for the virulence of IBDV, revealed no common amino acid substitutions among the pathogenic IBDVs in accordance with the propagation in DT40 cells. These results indicate that DT40 cells are a useful tool for rapid isolation of pathogenic field strains and successive in vitro analysis of IBDV.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalJournal of Veterinary Medical Science
Volume70
Issue number4
DOIs
Publication statusPublished - Apr 1 2008
Externally publishedYes

Keywords

  • DT40
  • IBDV
  • Virus isolation

ASJC Scopus subject areas

  • veterinary(all)

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