Abstract
The chemotactic specificity of Ia-positive and -negative macrophages was studied by using three macrophage chemotactic factors (MCF), -a, -b, and -c, isolated from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs. Listeria-elicited macrophages migrated toward MCF-a, -b, and -c. The chemotactic responses suggested responsive subpopulations to MCF. The electronic programmable individual cell sorter (EPICS) was used to separate macrophages with anti-Ia monoclonal antibodies. Ia-positive subpopulations responded to MCF-c, although they did not migrate toward MCF-a, and -b. In contrast, Ia-negative subpopulations migrated toward MCF-a and -b, but not toward MCF-c. Furthermore, MCF-c attracted Ia-positive macrophages, whereas MCF-a and -b were Ia-negative in vitro; MCF did not induce Ia-negative macrophages to express surface Ia-antigens in vitro. MCF-c was able to produce massive Ia-positive macrophage accumulations when injected i.p., whereas MCF-a accumulated Ia-negative macrophages. The data suggest that MCF-a and -b, which mediate initial macrophage reactions, attract Ia-negative macrophages, and that MCF-c, which mediates predominant macrophage reactions, attract Ia-positive macrophages in the DHR.
| Original language | English |
|---|---|
| Pages (from-to) | 2989-2994 |
| Number of pages | 6 |
| Journal | Journal of Immunology |
| Volume | 131 |
| Issue number | 6 |
| Publication status | Published - 1983 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Immunology
Cite this
Chemotactic macrophage subpopulations defined by macrophage chemotactic factors from delayed hypersensitivity reaction sites. / Honda, M.; Yoshimura, Teizo; Watanabe, T.; Hayashi, H.
In: Journal of Immunology, Vol. 131, No. 6, 1983, p. 2989-2994.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemotactic macrophage subpopulations defined by macrophage chemotactic factors from delayed hypersensitivity reaction sites
AU - Honda, M.
AU - Yoshimura, Teizo
AU - Watanabe, T.
AU - Hayashi, H.
PY - 1983
Y1 - 1983
N2 - The chemotactic specificity of Ia-positive and -negative macrophages was studied by using three macrophage chemotactic factors (MCF), -a, -b, and -c, isolated from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs. Listeria-elicited macrophages migrated toward MCF-a, -b, and -c. The chemotactic responses suggested responsive subpopulations to MCF. The electronic programmable individual cell sorter (EPICS) was used to separate macrophages with anti-Ia monoclonal antibodies. Ia-positive subpopulations responded to MCF-c, although they did not migrate toward MCF-a, and -b. In contrast, Ia-negative subpopulations migrated toward MCF-a and -b, but not toward MCF-c. Furthermore, MCF-c attracted Ia-positive macrophages, whereas MCF-a and -b were Ia-negative in vitro; MCF did not induce Ia-negative macrophages to express surface Ia-antigens in vitro. MCF-c was able to produce massive Ia-positive macrophage accumulations when injected i.p., whereas MCF-a accumulated Ia-negative macrophages. The data suggest that MCF-a and -b, which mediate initial macrophage reactions, attract Ia-negative macrophages, and that MCF-c, which mediates predominant macrophage reactions, attract Ia-positive macrophages in the DHR.
AB - The chemotactic specificity of Ia-positive and -negative macrophages was studied by using three macrophage chemotactic factors (MCF), -a, -b, and -c, isolated from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs. Listeria-elicited macrophages migrated toward MCF-a, -b, and -c. The chemotactic responses suggested responsive subpopulations to MCF. The electronic programmable individual cell sorter (EPICS) was used to separate macrophages with anti-Ia monoclonal antibodies. Ia-positive subpopulations responded to MCF-c, although they did not migrate toward MCF-a, and -b. In contrast, Ia-negative subpopulations migrated toward MCF-a and -b, but not toward MCF-c. Furthermore, MCF-c attracted Ia-positive macrophages, whereas MCF-a and -b were Ia-negative in vitro; MCF did not induce Ia-negative macrophages to express surface Ia-antigens in vitro. MCF-c was able to produce massive Ia-positive macrophage accumulations when injected i.p., whereas MCF-a accumulated Ia-negative macrophages. The data suggest that MCF-a and -b, which mediate initial macrophage reactions, attract Ia-negative macrophages, and that MCF-c, which mediates predominant macrophage reactions, attract Ia-positive macrophages in the DHR.
UR - http://www.scopus.com/inward/record.url?scp=0021068820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021068820&partnerID=8YFLogxK
M3 - Article
C2 - 6196408
AN - SCOPUS:0021068820
VL - 131
SP - 2989
EP - 2994
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -