Chemotactic macrophage subpopulations defined by macrophage chemotactic factors from delayed hypersensitivity reaction sites

M. Honda, T. Yoshimura, T. Watanabe, H. Hayashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The chemotactic specificity of Ia-positive and -negative macrophages was studied by using three macrophage chemotactic factors (MCF), -a, -b, and -c, isolated from delayed hypersensitivity reaction (DHR) skin sites in guinea pigs. Listeria-elicited macrophages migrated toward MCF-a, -b, and -c. The chemotactic responses suggested responsive subpopulations to MCF. The electronic programmable individual cell sorter (EPICS) was used to separate macrophages with anti-Ia monoclonal antibodies. Ia-positive subpopulations responded to MCF-c, although they did not migrate toward MCF-a, and -b. In contrast, Ia-negative subpopulations migrated toward MCF-a and -b, but not toward MCF-c. Furthermore, MCF-c attracted Ia-positive macrophages, whereas MCF-a and -b were Ia-negative in vitro; MCF did not induce Ia-negative macrophages to express surface Ia-antigens in vitro. MCF-c was able to produce massive Ia-positive macrophage accumulations when injected i.p., whereas MCF-a accumulated Ia-negative macrophages. The data suggest that MCF-a and -b, which mediate initial macrophage reactions, attract Ia-negative macrophages, and that MCF-c, which mediates predominant macrophage reactions, attract Ia-positive macrophages in the DHR.

Original languageEnglish
Pages (from-to)2989-2994
Number of pages6
JournalJournal of Immunology
Volume131
Issue number6
Publication statusPublished - Dec 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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