Chemokine production by human vascular smooth muscle cells: Modulation by IL-13

Nicola J. Jordan, Malcolm L. Watson, Robert J. Williams, Alan G. Roach, Teizo Yoshimura, John Westwick

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

1. The production of chemokines by vascular smooth muscle cells (SMC) is implicated in the pathogenesis of atherosclerosis, although the factors regulating chemokine production by these cells are incompletely characterized. 2. We describe the differential stimulation of interleukin-(IL)-8, monocyte chemoattractant protein (MCP)-1 and regulated on activation normal T-cell expressed and secreted (RANTES) synthesis following treatment of human vascular SMC with IL-α or tumour necrosis factor α (TNFα). Under basal conditions, cultured SMC release very low amounts of IL-8, MCP-1 and RANTES as assessed by specific ELISA. Concentration-response studies with IL-1α or TNFα revealed that each stimulus induced a similar amount of MCP-1. In contrast approximately three fold more IL-8 was induced by IL-1α than by TNFα whereas significant RANTES production was induced only by TNFα. These findings point to a divergence in the regulation of synthesis of the different chemokines in response to IL-1α or TNFα stimulation. 3. The T-cell derived cytokines IL-10 and IL-13 were also found to have differential effects on chemokine production by SMC. IL-13, but not IL-10, significantly enhanced IL-8 and MCP-1 release in response to IL-1α or TNFα. This increase in chemokine release appeared to be accounted for by increased mRNA expression. 4. These findings provide support for the concept that smooth muscle cells can have an active role in a local immune response via the production of chemokines which can be selectively modulated by T-cell derived cytokines.

Original languageEnglish
Pages (from-to)749-757
Number of pages9
JournalBritish Journal of Pharmacology
Volume122
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Interleukin-13
Vascular Smooth Muscle
Chemokines
Smooth Muscle Myocytes
Interleukin-8
Tumor Necrosis Factor-alpha
Chemokine CCL2
Interleukin-1
T-Lymphocytes
Interleukin-10
Cytokines
Atherosclerosis
Enzyme-Linked Immunosorbent Assay
Messenger RNA

Keywords

  • Chemokine
  • IL-10
  • IL-13
  • IL-8
  • Interleukin (IL)-1α
  • MCP-1
  • RANTES
  • Smooth muscle
  • TNFα

ASJC Scopus subject areas

  • Pharmacology

Cite this

Chemokine production by human vascular smooth muscle cells : Modulation by IL-13. / Jordan, Nicola J.; Watson, Malcolm L.; Williams, Robert J.; Roach, Alan G.; Yoshimura, Teizo; Westwick, John.

In: British Journal of Pharmacology, Vol. 122, No. 4, 1997, p. 749-757.

Research output: Contribution to journalArticle

Jordan, Nicola J. ; Watson, Malcolm L. ; Williams, Robert J. ; Roach, Alan G. ; Yoshimura, Teizo ; Westwick, John. / Chemokine production by human vascular smooth muscle cells : Modulation by IL-13. In: British Journal of Pharmacology. 1997 ; Vol. 122, No. 4. pp. 749-757.
@article{a733e5fdb8294c41b112592bf562e288,
title = "Chemokine production by human vascular smooth muscle cells: Modulation by IL-13",
abstract = "1. The production of chemokines by vascular smooth muscle cells (SMC) is implicated in the pathogenesis of atherosclerosis, although the factors regulating chemokine production by these cells are incompletely characterized. 2. We describe the differential stimulation of interleukin-(IL)-8, monocyte chemoattractant protein (MCP)-1 and regulated on activation normal T-cell expressed and secreted (RANTES) synthesis following treatment of human vascular SMC with IL-α or tumour necrosis factor α (TNFα). Under basal conditions, cultured SMC release very low amounts of IL-8, MCP-1 and RANTES as assessed by specific ELISA. Concentration-response studies with IL-1α or TNFα revealed that each stimulus induced a similar amount of MCP-1. In contrast approximately three fold more IL-8 was induced by IL-1α than by TNFα whereas significant RANTES production was induced only by TNFα. These findings point to a divergence in the regulation of synthesis of the different chemokines in response to IL-1α or TNFα stimulation. 3. The T-cell derived cytokines IL-10 and IL-13 were also found to have differential effects on chemokine production by SMC. IL-13, but not IL-10, significantly enhanced IL-8 and MCP-1 release in response to IL-1α or TNFα. This increase in chemokine release appeared to be accounted for by increased mRNA expression. 4. These findings provide support for the concept that smooth muscle cells can have an active role in a local immune response via the production of chemokines which can be selectively modulated by T-cell derived cytokines.",
keywords = "Chemokine, IL-10, IL-13, IL-8, Interleukin (IL)-1α, MCP-1, RANTES, Smooth muscle, TNFα",
author = "Jordan, {Nicola J.} and Watson, {Malcolm L.} and Williams, {Robert J.} and Roach, {Alan G.} and Teizo Yoshimura and John Westwick",
year = "1997",
doi = "10.1038/sj.bjp.0701433",
language = "English",
volume = "122",
pages = "749--757",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Chemokine production by human vascular smooth muscle cells

T2 - Modulation by IL-13

AU - Jordan, Nicola J.

AU - Watson, Malcolm L.

AU - Williams, Robert J.

AU - Roach, Alan G.

AU - Yoshimura, Teizo

AU - Westwick, John

PY - 1997

Y1 - 1997

N2 - 1. The production of chemokines by vascular smooth muscle cells (SMC) is implicated in the pathogenesis of atherosclerosis, although the factors regulating chemokine production by these cells are incompletely characterized. 2. We describe the differential stimulation of interleukin-(IL)-8, monocyte chemoattractant protein (MCP)-1 and regulated on activation normal T-cell expressed and secreted (RANTES) synthesis following treatment of human vascular SMC with IL-α or tumour necrosis factor α (TNFα). Under basal conditions, cultured SMC release very low amounts of IL-8, MCP-1 and RANTES as assessed by specific ELISA. Concentration-response studies with IL-1α or TNFα revealed that each stimulus induced a similar amount of MCP-1. In contrast approximately three fold more IL-8 was induced by IL-1α than by TNFα whereas significant RANTES production was induced only by TNFα. These findings point to a divergence in the regulation of synthesis of the different chemokines in response to IL-1α or TNFα stimulation. 3. The T-cell derived cytokines IL-10 and IL-13 were also found to have differential effects on chemokine production by SMC. IL-13, but not IL-10, significantly enhanced IL-8 and MCP-1 release in response to IL-1α or TNFα. This increase in chemokine release appeared to be accounted for by increased mRNA expression. 4. These findings provide support for the concept that smooth muscle cells can have an active role in a local immune response via the production of chemokines which can be selectively modulated by T-cell derived cytokines.

AB - 1. The production of chemokines by vascular smooth muscle cells (SMC) is implicated in the pathogenesis of atherosclerosis, although the factors regulating chemokine production by these cells are incompletely characterized. 2. We describe the differential stimulation of interleukin-(IL)-8, monocyte chemoattractant protein (MCP)-1 and regulated on activation normal T-cell expressed and secreted (RANTES) synthesis following treatment of human vascular SMC with IL-α or tumour necrosis factor α (TNFα). Under basal conditions, cultured SMC release very low amounts of IL-8, MCP-1 and RANTES as assessed by specific ELISA. Concentration-response studies with IL-1α or TNFα revealed that each stimulus induced a similar amount of MCP-1. In contrast approximately three fold more IL-8 was induced by IL-1α than by TNFα whereas significant RANTES production was induced only by TNFα. These findings point to a divergence in the regulation of synthesis of the different chemokines in response to IL-1α or TNFα stimulation. 3. The T-cell derived cytokines IL-10 and IL-13 were also found to have differential effects on chemokine production by SMC. IL-13, but not IL-10, significantly enhanced IL-8 and MCP-1 release in response to IL-1α or TNFα. This increase in chemokine release appeared to be accounted for by increased mRNA expression. 4. These findings provide support for the concept that smooth muscle cells can have an active role in a local immune response via the production of chemokines which can be selectively modulated by T-cell derived cytokines.

KW - Chemokine

KW - IL-10

KW - IL-13

KW - IL-8

KW - Interleukin (IL)-1α

KW - MCP-1

KW - RANTES

KW - Smooth muscle

KW - TNFα

UR - http://www.scopus.com/inward/record.url?scp=0030656243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030656243&partnerID=8YFLogxK

U2 - 10.1038/sj.bjp.0701433

DO - 10.1038/sj.bjp.0701433

M3 - Article

C2 - 9375973

AN - SCOPUS:0030656243

VL - 122

SP - 749

EP - 757

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 4

ER -