Abstract
We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
| Original language | English |
|---|---|
| Pages (from-to) | 1939-1952 |
| Number of pages | 14 |
| Journal | Life Sciences |
| Volume | 63 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - Oct 16 1998 |
| Externally published | Yes |
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Keywords
- Adhesion molecule
- Cytokine
- Interleukin- 8
- Monocyte chemotactic protein-1
- Neuroblastoma
ASJC Scopus subject areas
- Pharmacology
Cite this
Chemokine production and adhesion molecule expression by neural cells exposed to IL-1, TNFα and interferon. / Chuluyan, H. Eduardo; Lang, Bianca J.; Yoshimura, Teizo; Kenney, John S.; Issekutz, Andrew C.
In: Life Sciences, Vol. 63, No. 21, 16.10.1998, p. 1939-1952.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemokine production and adhesion molecule expression by neural cells exposed to IL-1, TNFα and interferon
AU - Chuluyan, H. Eduardo
AU - Lang, Bianca J.
AU - Yoshimura, Teizo
AU - Kenney, John S.
AU - Issekutz, Andrew C.
PY - 1998/10/16
Y1 - 1998/10/16
N2 - We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
AB - We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
KW - Adhesion molecule
KW - Cytokine
KW - Interleukin- 8
KW - Monocyte chemotactic protein-1
KW - Neuroblastoma
UR - http://www.scopus.com/inward/record.url?scp=0032538389&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032538389&partnerID=8YFLogxK
U2 - 10.1016/S0024-3205(98)00470-6
DO - 10.1016/S0024-3205(98)00470-6
M3 - Article
C2 - 9825772
AN - SCOPUS:0032538389
VL - 63
SP - 1939
EP - 1952
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 21
ER -