We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
- Adhesion molecule
- Interleukin- 8
- Monocyte chemotactic protein-1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)