Chemokine production and adhesion molecule expression by neural cells exposed to IL-1, TNFα and interferon

H. Eduardo Chuluyan, Bianca J. Lang, Teizo Yoshimura, John S. Kenney, Andrew C. Issekutz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.

Original languageEnglish
Pages (from-to)1939-1952
Number of pages14
JournalLife Sciences
Volume63
Issue number21
DOIs
Publication statusPublished - Oct 16 1998
Externally publishedYes

    Fingerprint

Keywords

  • Adhesion molecule
  • Cytokine
  • Interleukin- 8
  • Monocyte chemotactic protein-1
  • Neuroblastoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this