Chemoattractant efficacy: Oxidation of stimulus by responding cells

Teizo Yoshimura, Antal Rot, Edward J. Leonard

Research output: Contribution to journalArticle

Abstract

Although all human neutrophils have receptors for formyl-methionyl-leucyl-phenylalanine, only 20-30% migrate in vitro at optimal attractant concentrations. Since this attractant can be oxidized by myeloperoxidase from stimulated neutrophils, we determined if its efficacy was increased by protection from oxidation. Efficacy was increased by reducing agents and by molecules with oxidizable sulfur, 10-5M methionine or 1.5×10-6M albumin. The antioxidant effect was on the attractant, not the cells: albumin in the cell compartment did not increase responses. Furthermore, antioxidants had no effect on efficacy of fNle-Leu-Phe-Nle-Tyr-Lys, an attractant that also stimulated superoxide release but had no oxidizable sulfur.

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number1
DOIs
Publication statusPublished - Jul 16 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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