Chemiluminescence-HPLC assay of phosphatidylcholine hydroperoxide generated by ischemia-reperfusion in the liver of rats

Fusako Takayama, Toru Egashira, Yoshikuni Kudo, Yasumitsu Yamanaka

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To determine cellular damage due to "oxidative stress", we developed a sensitive and specific quantitative assay for phosphatidylcholine hydroperoxide (PCOOH) by coupling HPLC with detection of chemiluminescence (CL). The qualitative and quantitative detection limits of PCOOH by this assay were 0.5 and 2 pmol (based on active oxygen from hydroperoxide). Using this CL-HPLC method, we determined PCOOH levels caused by ischemia-reperfusion in rat livers. The PCOOH levels in livers of control, sham-operated and operated rats with only ischemic treatment were approximately 2 nmol/g wet liver weight. The PCOOH level and several serum parameters of liver injury increased with an increase in the duration of ischemia, and also increased in proportion to the duration of reperfusion. The determination of PCOOH in liver caused by ischemia-reperfusion could be a useful method for investigating liver damage induced by free radicals.

Original languageEnglish
Pages (from-to)2412-2414
Number of pages3
JournalBiochemical Pharmacology
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 15 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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