Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice

Hiroyuki Kawahigashi, Sakiko Hirose, Takayoshi Iwai, Yuko Ohashi, Wataru Sakamoto, Masahiko Maekawa, Yasunobu Ohkawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment.

Original languageEnglish
Pages (from-to)1241-1247
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number4
DOIs
Publication statusPublished - Feb 21 2007

Fingerprint

Anthocyanins
anthocyanins
promoter regions
genetically modified organisms
rice
Genetically Modified Plants
methyl jasmonate
Color
anthocyanidins
color
acids
gene expression regulation
Pigmentation
Gene Expression Regulation
chemical treatment
Reporter Genes
reporter genes
Gene expression
pigmentation
transgenic plants

Keywords

  • 2,6-dichloroisonicotinic acid (DCINA)
  • Anthocyanidin synthase
  • Biomonitoring
  • Methyl jasmonate
  • Oryza sativa
  • Phenylalanine ammonia lyase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice. / Kawahigashi, Hiroyuki; Hirose, Sakiko; Iwai, Takayoshi; Ohashi, Yuko; Sakamoto, Wataru; Maekawa, Masahiko; Ohkawa, Yasunobu.

In: Journal of Agricultural and Food Chemistry, Vol. 55, No. 4, 21.02.2007, p. 1241-1247.

Research output: Contribution to journalArticle

Kawahigashi, Hiroyuki ; Hirose, Sakiko ; Iwai, Takayoshi ; Ohashi, Yuko ; Sakamoto, Wataru ; Maekawa, Masahiko ; Ohkawa, Yasunobu. / Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice. In: Journal of Agricultural and Food Chemistry. 2007 ; Vol. 55, No. 4. pp. 1241-1247.
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