Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice

Hiroyuki Kawahigashi, Sakiko Hirose, Takayoshi Iwai, Yuko Ohashi, Wataru Sakamoto, Masahiko Maekawa, Yasunobu Ohkawa

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment.

    Original languageEnglish
    Pages (from-to)1241-1247
    Number of pages7
    JournalJournal of agricultural and food chemistry
    Volume55
    Issue number4
    DOIs
    Publication statusPublished - Feb 21 2007

    Keywords

    • 2,6-dichloroisonicotinic acid (DCINA)
    • Anthocyanidin synthase
    • Biomonitoring
    • Methyl jasmonate
    • Oryza sativa
    • Phenylalanine ammonia lyase

    ASJC Scopus subject areas

    • Chemistry(all)
    • Agricultural and Biological Sciences(all)

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