Chemical synthesis and expression of copper metallothionein gene of Neurospora crassa

Manabu Sugimoto, Tadao Oikawa, Nobuyoshi Esaki, Hidehiko Tanaka, Kenji Soda

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2 Citations (Scopus)

Abstract

The gene coding for the Neurospora crassa copper metallothionein (MT) was synthesized and inserted in the lacZ' gene of pUC18 plasmid to give the same translational reading frame as the latter gene. The MT-β-galactosidase fused gene was expressed in Escherichia coli to produce a fused protein in which the amino and carboxy termini of MT are linked to the β-galactosidase through methionine residues. An MT derivative containing an extra homoserine residue at the carboxy terminus was prepared by cyanogen bromide cleavage of the fused protein followed by a reverse-phase HPLC separation. The spectral features of the MT derivative and its copper complex were similar to those of the corresponding native MTs.

Original languageEnglish
Pages (from-to)924-926
Number of pages3
JournalJournal of biochemistry
Volume104
Issue number6
DOIs
Publication statusPublished - Dec 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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