Chemical structures of two subunits, A-subunit and B-subunit, of galactose-specific isolectins from Erythrina variegata seeds

Osamu Yamaguchi, Makoto Kimura, Masato Araki, Nobuyuki Yamasaki, Yoshinobu Kimura, Shuhei Nakajima, Shigeaki Takagi

Research output: Contribution to journalArticle

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Abstract

We previously isolated galactose-specific isolectins, EVLI, EVLII, and EVLUI, from the Erythrina variegata seeds [J. Chromatogr. 597, 207-211 (1992)]. The amino acid sequences of the two subunits, A- and B-subunits, of which the isolectins are composed, were determined. A comparison of the amino acid sequences of tryptic peptides from the two subunits revealed seven amino acid substitutions. Among them, Asn46, to which the oligosaccharide chain is linked in the A-subunit, is replaced by Asp46 in the B-subunit, causing the B-subunit to lack one glycosylation site. The N-linked oligosaccharides of these subunits were also analyzed. The N-linked oligosaccharides were first liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine, and then the pyridylamino (PA-) derivatives were purified by gel filtration and HPLC on an ODS-silica column. One major sugar chain, accounting for more than 98% of the total, was purified from both species. The structure of this major sugar chain was established to be Manα6(Manα3)(Xylβ2)Manβ4GlcNAcβ4(Fucα3)GlcNAc. This finding that there is no structural difference of the sugar chains linked to the two subunits of E. variegata lectins, together with the results of amino acid sequence comparisons, indicates that the difference in molecular mass of these two subunits results almost wholly from the difference in the number of oligosaccharides linked to them.

Original languageEnglish
Pages (from-to)560-566
Number of pages7
JournalJournal of Biochemistry
Volume114
Issue number4
Publication statusPublished - Oct 1993

Fingerprint

Erythrina
Oligosaccharides
Galactose
Lectins
Seed
Seeds
Amino acids
Amino Acid Sequence
Sugars
Amino Acids
Glycosylation
Lectin
Acetylation
Sequence Comparison
High-performance Liquid Chromatography
Molecular mass
Amino Acid Substitution
Silica
Peptides
Silicon Dioxide

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Yamaguchi, O., Kimura, M., Araki, M., Yamasaki, N., Kimura, Y., Nakajima, S., & Takagi, S. (1993). Chemical structures of two subunits, A-subunit and B-subunit, of galactose-specific isolectins from Erythrina variegata seeds. Journal of Biochemistry, 114(4), 560-566.

Chemical structures of two subunits, A-subunit and B-subunit, of galactose-specific isolectins from Erythrina variegata seeds. / Yamaguchi, Osamu; Kimura, Makoto; Araki, Masato; Yamasaki, Nobuyuki; Kimura, Yoshinobu; Nakajima, Shuhei; Takagi, Shigeaki.

In: Journal of Biochemistry, Vol. 114, No. 4, 10.1993, p. 560-566.

Research output: Contribution to journalArticle

Yamaguchi, O, Kimura, M, Araki, M, Yamasaki, N, Kimura, Y, Nakajima, S & Takagi, S 1993, 'Chemical structures of two subunits, A-subunit and B-subunit, of galactose-specific isolectins from Erythrina variegata seeds', Journal of Biochemistry, vol. 114, no. 4, pp. 560-566.
Yamaguchi, Osamu ; Kimura, Makoto ; Araki, Masato ; Yamasaki, Nobuyuki ; Kimura, Yoshinobu ; Nakajima, Shuhei ; Takagi, Shigeaki. / Chemical structures of two subunits, A-subunit and B-subunit, of galactose-specific isolectins from Erythrina variegata seeds. In: Journal of Biochemistry. 1993 ; Vol. 114, No. 4. pp. 560-566.
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