Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste

Benjamas Thanomsub, Wanna Pumeechockchai, Anirut Limtrakul, Panarat Arunrattiyakorn, Wipawan Petchleelaha, Teruhiko Nitoda, Hiroshi Kanzaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate or Rha-Rha-C10-C10 and l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 μg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 μg/mL.

Original languageEnglish
Pages (from-to)1149-1153
Number of pages5
JournalBioresource Technology
Volume98
Issue number5
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Bioactivity
factories
Pseudomonas aeruginosa
chemical structure
milk
surfactant
Industrial plants
bioactive properties
Milk
Surface active agents
Cells
Microbial Sensitivity Tests
minimum inhibitory concentration
Surface-Active Agents
surfactants
cell lines
biosurfactants
Cell Line
cancer
Polysorbates

Keywords

  • Anticancer
  • Biological activity
  • Biosurfactant
  • Glycolipid
  • Pseudomonas
  • Rhamnolipid

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. / Thanomsub, Benjamas; Pumeechockchai, Wanna; Limtrakul, Anirut; Arunrattiyakorn, Panarat; Petchleelaha, Wipawan; Nitoda, Teruhiko; Kanzaki, Hiroshi.

In: Bioresource Technology, Vol. 98, No. 5, 03.2007, p. 1149-1153.

Research output: Contribution to journalArticle

Thanomsub, Benjamas ; Pumeechockchai, Wanna ; Limtrakul, Anirut ; Arunrattiyakorn, Panarat ; Petchleelaha, Wipawan ; Nitoda, Teruhiko ; Kanzaki, Hiroshi. / Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. In: Bioresource Technology. 2007 ; Vol. 98, No. 5. pp. 1149-1153.
@article{12f2f68f926a45bfa88b9b5bf651d3d9,
title = "Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste",
abstract = "The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate or Rha-Rha-C10-C10 and l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 μg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 μg/mL.",
keywords = "Anticancer, Biological activity, Biosurfactant, Glycolipid, Pseudomonas, Rhamnolipid",
author = "Benjamas Thanomsub and Wanna Pumeechockchai and Anirut Limtrakul and Panarat Arunrattiyakorn and Wipawan Petchleelaha and Teruhiko Nitoda and Hiroshi Kanzaki",
year = "2007",
month = "3",
doi = "10.1016/j.biortech.2005.10.045",
language = "English",
volume = "98",
pages = "1149--1153",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste

AU - Thanomsub, Benjamas

AU - Pumeechockchai, Wanna

AU - Limtrakul, Anirut

AU - Arunrattiyakorn, Panarat

AU - Petchleelaha, Wipawan

AU - Nitoda, Teruhiko

AU - Kanzaki, Hiroshi

PY - 2007/3

Y1 - 2007/3

N2 - The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate or Rha-Rha-C10-C10 and l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 μg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 μg/mL.

AB - The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate or Rha-Rha-C10-C10 and l-rhamnopyranosyl-l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 μg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 μg/mL.

KW - Anticancer

KW - Biological activity

KW - Biosurfactant

KW - Glycolipid

KW - Pseudomonas

KW - Rhamnolipid

UR - http://www.scopus.com/inward/record.url?scp=33750294814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750294814&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2005.10.045

DO - 10.1016/j.biortech.2005.10.045

M3 - Article

C2 - 16781144

AN - SCOPUS:33750294814

VL - 98

SP - 1149

EP - 1153

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

IS - 5

ER -