Chemical modification of Vibrio vulnificus metalloprotease with activated polyethylene glycol

Hitoshi Narukawa, Shin-ichi Miyoshi, Sumio Shinoda

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Abstract

Vibrio vulnificus, an opportunistic human pathogen causing septicemia, produces a metalloprotease which is suspected to be a virulence determinant, but which is labile in vivo due to inactivation by α-macroglobulin. To obtain a derivative which is stable in vivo, the metalloprotease was modified with activated monomethoxy polyethylene glycol. The modified protease retained full activity to a peptide substrate and 10-20% activity to protein substrates, and was resistant to entrapment by α-macroglobulin because of the increased molecular size (approx. 90 kDa). These findings suggest that the modified protease is stable in vivo and may be used to investigate the pathological actions of the protease in the bloodstream.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalFEMS Microbiology Letters
Volume108
Issue number1
Publication statusPublished - Mar 15 1993

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Keywords

  • Bacterial metalloprotease
  • Chemical modification
  • Polyethylene glycol
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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