Chemical modification of guanine residues of mouse 5 S ribosomal RNA with kethoxal. Nucleosides and nucleotides 46

Kazunobu Miura, Shigeki Tsuda, Tohru Ueda, Fumio Harada, Nobuyuki Kato

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Chemical modification of mouse 5 S rRNA with kethoxal was carried out to examine the secondary structure. The guanine residues located at positions 37, 41, 56, 66, 75 and 89 were modified. The relative rates of reaction are in the order G37, G56, G89, G66, G41, G75 at 28°C and G37, G41, G56, G89, G75, G66 at 35°C. These results support a secondary structure model containing 5 helices and 5 loops and indicate that the region around position 37 is the most exposed in higher-order structure.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalBBA - Gene Structure and Expression
Issue number3
Publication statusPublished - Apr 15 1983
Externally publishedYes



  • (Mouse kidney)
  • Chemical modification
  • Guanine residue
  • Kethoxal
  • ribosomal RNA
  • Secondary structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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