TY - JOUR
T1 - Chemical modification of guanine residues of mouse 5 S ribosomal RNA with kethoxal. Nucleosides and nucleotides 46
AU - Miura, Kazunobu
AU - Tsuda, Shigeki
AU - Ueda, Tohru
AU - Harada, Fumio
AU - Kato, Nobuyuki
N1 - Funding Information:
This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1983/4/15
Y1 - 1983/4/15
N2 - Chemical modification of mouse 5 S rRNA with kethoxal was carried out to examine the secondary structure. The guanine residues located at positions 37, 41, 56, 66, 75 and 89 were modified. The relative rates of reaction are in the order G37, G56, G89, G66, G41, G75 at 28°C and G37, G41, G56, G89, G75, G66 at 35°C. These results support a secondary structure model containing 5 helices and 5 loops and indicate that the region around position 37 is the most exposed in higher-order structure.
AB - Chemical modification of mouse 5 S rRNA with kethoxal was carried out to examine the secondary structure. The guanine residues located at positions 37, 41, 56, 66, 75 and 89 were modified. The relative rates of reaction are in the order G37, G56, G89, G66, G41, G75 at 28°C and G37, G41, G56, G89, G75, G66 at 35°C. These results support a secondary structure model containing 5 helices and 5 loops and indicate that the region around position 37 is the most exposed in higher-order structure.
KW - (Mouse kidney)
KW - Chemical modification
KW - Guanine residue
KW - Kethoxal
KW - Secondary structure
KW - ribosomal RNA
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U2 - 10.1016/0167-4781(83)90102-1
DO - 10.1016/0167-4781(83)90102-1
M3 - Article
C2 - 6403036
AN - SCOPUS:0020582965
SN - 0167-4781
VL - 739
SP - 281
EP - 285
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -