Chemical Modification of Cytosine Residues of tRNAVal with Hydrogen Sulfide (Nucleosides and Nucleotides. XL)

Kazunobu Miura, Tatsuyoshi Iwano, Shigeki Tsuda, Tohru Ueda, Fumio Harada, Nobuyuki Kato

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The conversion of cytosine residue of transfer ribonucleic acid (tRNA) to 4-thiouracil residues was carried out with a hydrogen sulfide-pyridine-water system. In the case of tRNAVal from mouse cells, the cytosine moieties located in unpaired and looped-out regions of the molecule, such as the anticodon and CCA-end regions, were converted. This result shows that the secondary and tertiary structures of tRNA are maintained even in medium containing liquid hydrogen sulfide and pyridine, and hence, this system should be suitable for studies of the higher-order structure of nucleic acids.

Original languageEnglish
Pages (from-to)4126-4133
Number of pages8
JournalChemical and Pharmaceutical Bulletin
Issue number11
Publication statusPublished - Jan 1 1982
Externally publishedYes


  • 4-thiouracil residue
  • Sulfhydrolysis
  • chemical modification
  • hydrogen sulfide
  • modification of tRNA
  • mouse cells-tRNA

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery


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