Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)

Kazunobu Miura, Shigeki Tsuda, Tatsuyoshi Iwano, Tohru Ueda, Fumio Harada, Nobuyuki Kato

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28°C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35°C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.

Original languageEnglish
Pages (from-to)181-189
Number of pages9
JournalBBA - Gene Structure and Expression
Volume739
Issue number2
DOIs
Publication statusPublished - Mar 10 1983
Externally publishedYes

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Keywords

  • (Mouse kidney)
  • Chemical modification
  • Cytosine
  • Hydrogen sulfide
  • Thiouracil
  • rRNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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