Chemical genetics reveals negative regulation of abscisic acid signaling by a plant immune response pathway

Tae Houn Kim, Felix Hauser, Tracy Ha, Shaowu Xue, Maik Böhmer, Noriyuki Nishimura, Shintaro Munemasa, Katharine Hubbard, Nora Peine, Byeong Ha Lee, Stephen Lee, Nadia Robert, Jane E. Parker, Julian I. Schroeder

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Coordinated regulation of protection mechanisms against environmental abiotic stress and pathogen attack is essential for plant adaptation and survival. Initial abiotic stress can interfere with disease-resistance signaling [1-6]. Conversely, initial plant immune signaling may interrupt subsequent abscisic acid (ABA) signal transduction [7, 8]. However, the processes involved in this crosstalk between these signaling networks have not been determined. By screening a 9600-compound chemical library, we identified a small molecule [5-(3,4-dichlorophenyl)furan-2-yl]-piperidine-1-ylmethanethione (DFPM) that rapidly downregulates ABA-dependent gene expression and also inhibits ABA-induced stomatal closure. Transcriptome analyses show that DFPM also stimulates expression of plant defense-related genes. Major early regulators of pathogen-resistance responses, including EDS1, PAD4, RAR1, and SGT1b, are required for DFPM - and notably also for Pseudomonas - interference with ABA signal transduction, whereas salicylic acid, EDS16, and NPR1 are not necessary. Although DFPM does not interfere with early ABA perception by PYR/RCAR receptors or ABA activation of SnRK2 kinases, it disrupts cytosolic Ca2+ signaling and downstream anion channel activation in a PAD4-dependent manner. Our findings provide evidence that activation of EDS1/PAD4-dependent plant immune responses rapidly disrupts ABA signal transduction and that this occurs at the level of Ca2+ signaling, illuminating how the initial biotic stress pathway interferes with ABA signaling.

Original languageEnglish
Pages (from-to)990-997
Number of pages8
JournalCurrent Biology
Volume21
Issue number11
DOIs
Publication statusPublished - Jun 7 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Chemical genetics reveals negative regulation of abscisic acid signaling by a plant immune response pathway'. Together they form a unique fingerprint.

  • Cite this

    Kim, T. H., Hauser, F., Ha, T., Xue, S., Böhmer, M., Nishimura, N., Munemasa, S., Hubbard, K., Peine, N., Lee, B. H., Lee, S., Robert, N., Parker, J. E., & Schroeder, J. I. (2011). Chemical genetics reveals negative regulation of abscisic acid signaling by a plant immune response pathway. Current Biology, 21(11), 990-997. https://doi.org/10.1016/j.cub.2011.04.045