Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino)

Bei Wei Zhu, Da yong Zhou, Tao Li, Shuo Yan, Jing feng Yang, Dong mei Li, Xiu ping Dong, Yoshiyuki Murata

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Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PWXL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).

Original languageEnglish
Pages (from-to)712-718
Number of pages7
JournalFood Chemistry
Issue number3
Publication statusPublished - Aug 1 2010



  • Chemical composition
  • Free radical scavenging activity
  • Gonad
  • Haliotis Discus Hannai Ino
  • Sulphated Polysaccharide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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