Abstract
Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PWXL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).
| Original language | English |
|---|---|
| Pages (from-to) | 712-718 |
| Number of pages | 7 |
| Journal | Food Chemistry |
| Volume | 121 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 1 2010 |
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Keywords
- Chemical composition
- Free radical scavenging activity
- Gonad
- Haliotis Discus Hannai Ino
- Sulphated Polysaccharide
ASJC Scopus subject areas
- Food Science
- Analytical Chemistry
Cite this
Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino). / Zhu, Bei Wei; Zhou, Da yong; Li, Tao; Yan, Shuo; Yang, Jing feng; Li, Dong mei; Dong, Xiu ping; Murata, Yoshiyuki.
In: Food Chemistry, Vol. 121, No. 3, 01.08.2010, p. 712-718.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino)
AU - Zhu, Bei Wei
AU - Zhou, Da yong
AU - Li, Tao
AU - Yan, Shuo
AU - Yang, Jing feng
AU - Li, Dong mei
AU - Dong, Xiu ping
AU - Murata, Yoshiyuki
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PWXL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).
AB - Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PWXL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).
KW - Chemical composition
KW - Free radical scavenging activity
KW - Gonad
KW - Haliotis Discus Hannai Ino
KW - Sulphated Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=77649191501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77649191501&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2010.01.010
DO - 10.1016/j.foodchem.2010.01.010
M3 - Article
AN - SCOPUS:77649191501
VL - 121
SP - 712
EP - 718
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
IS - 3
ER -