Characterization of Xenopus digits and regenerated limbs of the froglet

Akira Satoh, Tetsuya Endo, Masahiro Abe, Nayuta Yakushiji, Shiro Ohgo, Koji Tamura, Hiroyuki Ide

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Xenopus has 4 and 5 digits in a forelimb and hindlimb, respectively. It is thought that their limbs and digits develop in Xenopus by mechanisms that are almost conserved from amphibians to higher vertebrates. This is supported by some molecular evidence. The 5'hoxd genes are convenient marker genes for characterizing digits in the chick and mouse. The anteriormost digit is characterized by being hoxd13-positive and hoxd12 (hoxd11)-negative in the chick and mouse. In this study, we revealed that the anteriormost digit of the Xenopus forelimb is hoxd13-positive and hoxd11-positive, that is, a more posterior character than digit I. The order of formation of digit cartilages also suggested that Xenopus forelimb digit identity is II to V, not I to IV. We have also been interested in the relationship between digit identity and shh. The anteriormost digit develops in a shh-independent way. A limb treated with cyclopamine (a shh inhibitor) has a gene expression pattern (hoxd11-negative) similar to that in shh-deficient mice, suggesting that a hindlimb treated with cyclopamine has a digit I character. However, a Xenopus froglet regenerate (spike), which lacks shh expression during its regeneration process, does not have such an expression pattern, being hoxd11-positive. We investigated hoxd11 transcriptions in blastemas that formed in the anteriormost and posteriormost digits, and we found that the blastemas have different hoxd11 expression levels. These findings suggest that the froglet limb blastema does not have a mere digit I character in spite of shh defectiveness and that the froglet limb blastema recognizes its positional differences along the anterior-posterior axis.

Original languageEnglish
Pages (from-to)3316-3326
Number of pages11
JournalDevelopmental Dynamics
Volume235
Issue number12
DOIs
Publication statusPublished - Dec 1 2006
Externally publishedYes

Keywords

  • Digit identity
  • Limb
  • Regeneration and spike
  • Xenopus
  • hoxd11

ASJC Scopus subject areas

  • Developmental Biology

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  • Cite this

    Satoh, A., Endo, T., Abe, M., Yakushiji, N., Ohgo, S., Tamura, K., & Ide, H. (2006). Characterization of Xenopus digits and regenerated limbs of the froglet. Developmental Dynamics, 235(12), 3316-3326. https://doi.org/10.1002/dvdy.20985