Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species

Kiyotaka Nagaki, Kazunari Kashihara, Minoru Murata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.

Original languageEnglish
Pages (from-to)719-726
Number of pages8
JournalChromosome Research
Volume17
Issue number6
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Protein C
Tobacco
Centromere
Kinetochores
Tetraploidy
Eukaryota
Histones
Proteins
Complementary DNA
Chromosomes
centromere protein C
DNA
Genes

Keywords

  • CENP-C
  • Centromere
  • MIS12
  • Nicotiana
  • Tobacco

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species. / Nagaki, Kiyotaka; Kashihara, Kazunari; Murata, Minoru.

In: Chromosome Research, Vol. 17, No. 6, 08.2009, p. 719-726.

Research output: Contribution to journalArticle

Nagaki, Kiyotaka ; Kashihara, Kazunari ; Murata, Minoru. / Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species. In: Chromosome Research. 2009 ; Vol. 17, No. 6. pp. 719-726.
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