Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis aTCC 33277

Tetsuyoshi Inoue, Masaaki Nakayama, Yuko Taguchi, Konami Kano, Miyuu Ono, Yurika Shimizu, Teruo Kuroda, Naoya Oohara

Research output: Contribution to journalArticle

Abstract

The periodontal pathogen, Porphyromonas gingivalis ATCC 33277 has six gene clusters that encode tripartite drug efflux pumps. To examine the effects of the drug efflux pumps on its antibiotic sensitivity, six mutants were constructed, each defective in the membrane fusion protein gene of each gene cluster. Compared to the wild-type strain, all mutants exhibited an elevated sensitivity to tetracycline, and two mutants with deletions in the PGN_1431 and PGN_1680 genes showed an increased sensitivity to various types of antibiotics. These results suggest that the activity of drug efflux systems may affect antibiotic sensitivity in P. gingivalis.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalNew Microbiologica
Volume38
Issue number1
Publication statusPublished - Jan 1 2015

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Porphyromonas gingivalis
Multigene Family
Anti-Bacterial Agents
Membrane Fusion Proteins
Pharmaceutical Preparations
Tetracycline
Genes

Keywords

  • Antibiotic sensitivity
  • Drug efflux pump
  • Membrane fusion protein
  • Phylogenetic tree
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis aTCC 33277. / Inoue, Tetsuyoshi; Nakayama, Masaaki; Taguchi, Yuko; Kano, Konami; Ono, Miyuu; Shimizu, Yurika; Kuroda, Teruo; Oohara, Naoya.

In: New Microbiologica, Vol. 38, No. 1, 01.01.2015, p. 101-108.

Research output: Contribution to journalArticle

Inoue, T, Nakayama, M, Taguchi, Y, Kano, K, Ono, M, Shimizu, Y, Kuroda, T & Oohara, N 2015, 'Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis aTCC 33277', New Microbiologica, vol. 38, no. 1, pp. 101-108.
Inoue T, Nakayama M, Taguchi Y, Kano K, Ono M, Shimizu Y et al. Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis aTCC 33277. New Microbiologica. 2015 Jan 1;38(1):101-108.
Inoue, Tetsuyoshi ; Nakayama, Masaaki ; Taguchi, Yuko ; Kano, Konami ; Ono, Miyuu ; Shimizu, Yurika ; Kuroda, Teruo ; Oohara, Naoya. / Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis aTCC 33277. In: New Microbiologica. 2015 ; Vol. 38, No. 1. pp. 101-108.
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