Characterization of the tripartite drug efflux pumps of Porphyromonas gingivalis ATCC 33277

Tetsuyoshi Inoue, Masaaki Nakayama, Yuko Taguchi, Konami Kano, Miyuu Ono, Yurika Shimizu, Teruo Kuroda, Naoya Ohara

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The periodontal pathogen, Porphyromonas gingivalis ATCC 33277 has six gene clusters that encode tripartite drug efflux pumps. To examine the effects of the drug efflux pumps on its antibiotic sensitivity, six mutants were constructed, each defective in the membrane fusion protein gene of each gene cluster. Compared to the wild-type strain, all mutants exhibited an elevated sensitivity to tetracycline, and two mutants with deletions in the PGN_1431 and PGN_1680 genes showed an increased sensitivity to various types of antibiotics. These results suggest that the activity of drug efflux systems may affect antibiotic sensitivity in P. gingivalis.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalNew Microbiologica
Volume38
Issue number1
Publication statusPublished - Jan 1 2015

Keywords

  • Antibiotic sensitivity
  • Drug efflux pump
  • Membrane fusion protein
  • Phylogenetic tree
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Microbiology (medical)

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