Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Kirara Saito; Megumi Watanabe; Hidenori Matsui; Mikihiro Yamamoto; Yuki Ichinose; Kazuhiro Toyoda; Akira Kawaguchi; Yoshiteru Noutoshi

doi:10.1007/s10327-017-0756-1

Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Kirara Saito, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Akira Kawaguchi, Yoshiteru Noutoshi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of General Plant Pathology
DOIs	https://doi.org/10.1007/s10327-017-0756-1
Publication status	Accepted/In press - Dec 2 2017

Keywords

Antitumorigenic bacterial strain
Biological control agent
Crown gall disease
Rhizobium vitis

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

Access to Document

10.1007/s10327-017-0756-1

Fingerprint Dive into the research topics of 'Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis'. Together they form a unique fingerprint.

Cite this

Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis. / Saito, Kirara; Watanabe, Megumi; Matsui, Hidenori ; Yamamoto, Mikihiro ; Ichinose, Yuki ; Toyoda, Kazuhiro; Kawaguchi, Akira; Noutoshi, Yoshiteru.

In: Journal of General Plant Pathology, 02.12.2017, p. 1-7.

Research output: Contribution to journal › Article › peer-review

@article{c25a5284e5d946dc8dff915039e0ca72,

title = "Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis",

abstract = "Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.",

keywords = "Antitumorigenic bacterial strain, Biological control agent, Crown gall disease, Rhizobium vitis",

author = "Kirara Saito and Megumi Watanabe and Hidenori Matsui and Mikihiro Yamamoto and Yuki Ichinose and Kazuhiro Toyoda and Akira Kawaguchi and Yoshiteru Noutoshi",

year = "2017",

month = dec,

day = "2",

doi = "10.1007/s10327-017-0756-1",

language = "English",

pages = "1--7",

journal = "Journal of General Plant Pathology",

issn = "1345-2630",

publisher = "Springer Japan",

}

TY - JOUR

T1 - Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

AU - Saito, Kirara

AU - Watanabe, Megumi

AU - Matsui, Hidenori

AU - Yamamoto, Mikihiro

AU - Ichinose, Yuki

AU - Toyoda, Kazuhiro

AU - Kawaguchi, Akira

AU - Noutoshi, Yoshiteru

PY - 2017/12/2

Y1 - 2017/12/2

N2 - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

AB - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

KW - Antitumorigenic bacterial strain

KW - Biological control agent

KW - Crown gall disease

KW - Rhizobium vitis

UR - http://www.scopus.com/inward/record.url?scp=85035796897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035796897&partnerID=8YFLogxK

U2 - 10.1007/s10327-017-0756-1

DO - 10.1007/s10327-017-0756-1

M3 - Article

AN - SCOPUS:85035796897

SP - 1

EP - 7

JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

SN - 1345-2630

ER -