Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Kirara Saito, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Akira Kawaguchi, Yoshiteru Noutoshi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of General Plant Pathology
DOIs
Publication statusAccepted/In press - Dec 2 2017

Fingerprint

Rhizobium vitis
biological control
virulence
acetosyringone
crown galls
filtrates
culture filtrates
biological control agents
culture media
heat treatment
molecular weight
genes

Keywords

  • Antitumorigenic bacterial strain
  • Biological control agent
  • Crown gall disease
  • Rhizobium vitis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis",
abstract = "Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.",
keywords = "Antitumorigenic bacterial strain, Biological control agent, Crown gall disease, Rhizobium vitis",
author = "Kirara Saito and Megumi Watanabe and Hidenori Matsui and Mikihiro Yamamoto and Yuki Ichinose and Kazuhiro Toyoda and Akira Kawaguchi and Yoshiteru Noutoshi",
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T1 - Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

AU - Saito, Kirara

AU - Watanabe, Megumi

AU - Matsui, Hidenori

AU - Yamamoto, Mikihiro

AU - Ichinose, Yuki

AU - Toyoda, Kazuhiro

AU - Kawaguchi, Akira

AU - Noutoshi, Yoshiteru

PY - 2017/12/2

Y1 - 2017/12/2

N2 - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

AB - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

KW - Antitumorigenic bacterial strain

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KW - Rhizobium vitis

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