Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Kirara Saito; Megumi Watanabe; Hidenori Matsui; Mikihiro Yamamoto; Yuki Ichinose; Kazuhiro Toyoda; Akira Kawaguchi; Yoshiteru Noutoshi

doi:10.1007/s10327-017-0756-1

Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Kirara Saito, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Akira Kawaguchi, Yoshiteru Noutoshi

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

Original language	English
Pages (from-to)	58-64
Number of pages	7
Journal	Journal of General Plant Pathology
Volume	84
Issue number	1
DOIs	https://doi.org/10.1007/s10327-017-0756-1
Publication status	Published - Jan 1 2018

Keywords

Antitumorigenic bacterial strain
Biological control agent
Crown gall disease
Rhizobium vitis

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-017-0756-1

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title = "Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis",

abstract = "Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.",

keywords = "Antitumorigenic bacterial strain, Biological control agent, Crown gall disease, Rhizobium vitis",

author = "Kirara Saito and Megumi Watanabe and Hidenori Matsui and Mikihiro Yamamoto and Yuki Ichinose and Kazuhiro Toyoda and Akira Kawaguchi and Yoshiteru Noutoshi",

note = "Funding Information: Acknowledgements The bacterial strains were kindly provided by Okayama Prefecture in Japan via the dedicated arrangement of a material transfer agreement by Mr. Koji Tanina. This research was supported by KAKENHI Grant 17H03778 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A.K. and Y.N., and basic funds from Okayama University to Y.N. We also thank Honokai for providing a scholarship to K.S. Publisher Copyright: {\textcopyright} 2017, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.",

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doi = "10.1007/s10327-017-0756-1",

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T1 - Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

AU - Saito, Kirara

AU - Watanabe, Megumi

AU - Matsui, Hidenori

AU - Yamamoto, Mikihiro

AU - Ichinose, Yuki

AU - Toyoda, Kazuhiro

AU - Kawaguchi, Akira

AU - Noutoshi, Yoshiteru

N1 - Funding Information: Acknowledgements The bacterial strains were kindly provided by Okayama Prefecture in Japan via the dedicated arrangement of a material transfer agreement by Mr. Koji Tanina. This research was supported by KAKENHI Grant 17H03778 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A.K. and Y.N., and basic funds from Okayama University to Y.N. We also thank Honokai for providing a scholarship to K.S. Publisher Copyright: © 2017, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

AB - Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

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Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R. vitis

Abstract

Keywords

ASJC Scopus subject areas

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