Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies

Takayuki Tabayashi, Fumihiko Ishimaru, Minoru Takata, Itaru Kataoka, Koichi Nakase, Teruhiko Kozuka, Mitsune Tanimoto

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Abstract

In an earlier report, we demonstrated overexpression of a short isoform of Helios, Hel-5, which lacks three of four N-terminal zinc fingers, in patients with adult T-cell leukemia/lymphoma. Here, we characterized Hel-5 using immunoprecipitation, and gel shift and luciferase promoter assays, and found that Hel-5 lacks the repressor function observed with a full-length isoform of Helios. Moreover, Hel-5 associates with the full-length isoforms of the Ikaros gene family, Ikaros, Aiolos and Helios, and inhibits their DNA binding activity when present in excess, leading to dominant-negative effects on the full-length isoforms of the Ikaros gene family. Our results suggest a critical role for Helios in the mechanism of leukemogenesis.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalCancer Science
Volume98
Issue number2
DOIs
Publication statusPublished - Feb 2007

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tabayashi, T., Ishimaru, F., Takata, M., Kataoka, I., Nakase, K., Kozuka, T., & Tanimoto, M. (2007). Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies. Cancer Science, 98(2), 182-188. https://doi.org/10.1111/j.1349-7006.2006.00372.x