Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies

Takayuki Tabayashi, Fumihiko Ishimaru, Minoru Takata, Itaru Kataoka, Koichi Nakase, Teruhiko Kozuka, Mitsune Tanimoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In an earlier report, we demonstrated overexpression of a short isoform of Helios, Hel-5, which lacks three of four N-terminal zinc fingers, in patients with adult T-cell leukemia/lymphoma. Here, we characterized Hel-5 using immunoprecipitation, and gel shift and luciferase promoter assays, and found that Hel-5 lacks the repressor function observed with a full-length isoform of Helios. Moreover, Hel-5 associates with the full-length isoforms of the Ikaros gene family, Ikaros, Aiolos and Helios, and inhibits their DNA binding activity when present in excess, leading to dominant-negative effects on the full-length isoforms of the Ikaros gene family. Our results suggest a critical role for Helios in the mechanism of leukemogenesis.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalCancer Science
Volume98
Issue number2
DOIs
Publication statusPublished - Feb 2007

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Protein Isoforms
T-Lymphocytes
Neoplasms
Adult T Cell Leukemia Lymphoma
Zinc Fingers
Luciferases
Immunoprecipitation
Genes
Gels
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tabayashi, T., Ishimaru, F., Takata, M., Kataoka, I., Nakase, K., Kozuka, T., & Tanimoto, M. (2007). Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies. Cancer Science, 98(2), 182-188. https://doi.org/10.1111/j.1349-7006.2006.00372.x

Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies. / Tabayashi, Takayuki; Ishimaru, Fumihiko; Takata, Minoru; Kataoka, Itaru; Nakase, Koichi; Kozuka, Teruhiko; Tanimoto, Mitsune.

In: Cancer Science, Vol. 98, No. 2, 02.2007, p. 182-188.

Research output: Contribution to journalArticle

Tabayashi, T, Ishimaru, F, Takata, M, Kataoka, I, Nakase, K, Kozuka, T & Tanimoto, M 2007, 'Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies', Cancer Science, vol. 98, no. 2, pp. 182-188. https://doi.org/10.1111/j.1349-7006.2006.00372.x
Tabayashi, Takayuki ; Ishimaru, Fumihiko ; Takata, Minoru ; Kataoka, Itaru ; Nakase, Koichi ; Kozuka, Teruhiko ; Tanimoto, Mitsune. / Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies. In: Cancer Science. 2007 ; Vol. 98, No. 2. pp. 182-188.
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