Characterization of the regulation of ethylene biosynthesis in tomato fruit by carbon dioxide and diazocyclopentadiene

Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The regulation of ethylene biosynthesis by CO2 and diazocyclopentadiene (DACP), both inhibitors of ethylene action, was investigated in tomato (Lycopersicon esculentum Mill. cv. 'Momotaro') fruit held at 25 °C. When the tomato fruit at the pink stage of ripeness were treated with 20% CO2 (+ 20% O2 + 60% N2) or DACP, ethylene production by the fruit was rapidly decreased. The inhibition of ethylene production resulted primarily, if not solely, from the suppression of the activities of both 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. The inhibition of ACC synthase activity subsequently led to low levels of ACC. CO2 treatment further inhibited ACC conjugation into 1-(malonylamino) cyclopropane-1-carboxylic acid (MACC). By contrast, DACP-treated fruit maintained slightly higher levels of MACC relative to the control fruit. When the fruit were transferred from the CO2-enriched atmosphere to air, ethylene production, ACC and MACC contents and the activities of ACC synthase and ACC oxidase increased gradually to the control level after 24 h, while these values, except for MACC content, remained low in DACP-treated fruit throughout the experimental period. These results indicate that CO2 and DACP regulate ethylene production in tomato fruit by inhibiting ACC synthase and ACC oxidase activities and further support the hypothesis that the autocatalytic signal associated with ethylene action during fruit ripening stimulates the activities of both enzymes.

Original languageEnglish
Pages (from-to)221-233
Number of pages13
JournalPostharvest Biology and Technology
Volume5
Issue number3
DOIs
Publication statusPublished - 1995

Fingerprint

Lycopersicon esculentum
Carbon Dioxide
ethylene production
1-aminocyclopropane-1-carboxylic acid
Fruit
carbon dioxide
tomatoes
fruits
carboxylic acids
1-aminocyclopropane-1-carboxylate synthase
Oxidoreductases
ethylene
ethylene inhibitors
Solanum lycopersicum var. lycopersicum
Atmosphere
ripening
enzyme activity
Air
air
cyclopropanecarboxylic acid

Keywords

  • Carbon dioxide
  • Diazocyclopentadiene
  • Ethylene biosynthesis
  • Lycopersicon esculentum
  • Tomato

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Horticulture

Cite this

Characterization of the regulation of ethylene biosynthesis in tomato fruit by carbon dioxide and diazocyclopentadiene. / Mathooko, Francis M.; Kubo, Yasutaka; Inaba, Akitsugu; Nakamura, Reinosuke.

In: Postharvest Biology and Technology, Vol. 5, No. 3, 1995, p. 221-233.

Research output: Contribution to journalArticle

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AB - The regulation of ethylene biosynthesis by CO2 and diazocyclopentadiene (DACP), both inhibitors of ethylene action, was investigated in tomato (Lycopersicon esculentum Mill. cv. 'Momotaro') fruit held at 25 °C. When the tomato fruit at the pink stage of ripeness were treated with 20% CO2 (+ 20% O2 + 60% N2) or DACP, ethylene production by the fruit was rapidly decreased. The inhibition of ethylene production resulted primarily, if not solely, from the suppression of the activities of both 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. The inhibition of ACC synthase activity subsequently led to low levels of ACC. CO2 treatment further inhibited ACC conjugation into 1-(malonylamino) cyclopropane-1-carboxylic acid (MACC). By contrast, DACP-treated fruit maintained slightly higher levels of MACC relative to the control fruit. When the fruit were transferred from the CO2-enriched atmosphere to air, ethylene production, ACC and MACC contents and the activities of ACC synthase and ACC oxidase increased gradually to the control level after 24 h, while these values, except for MACC content, remained low in DACP-treated fruit throughout the experimental period. These results indicate that CO2 and DACP regulate ethylene production in tomato fruit by inhibiting ACC synthase and ACC oxidase activities and further support the hypothesis that the autocatalytic signal associated with ethylene action during fruit ripening stimulates the activities of both enzymes.

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