Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Yuuka Kimura, Tomoaki Kawakami, Long Jiang Yu, Miku Yoshimura, Masayuki Kobayashi, Zheng Yu Wang-Otomo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

Original languageEnglish
Article number37199
Pages (from-to)1761-1765
Number of pages5
JournalFEBS Letters
Volume589
Issue number15
DOIs
Publication statusPublished - Jul 3 2015

Keywords

  • Chromatophore
  • Electron transport
  • Light-harvesting
  • Photosynthesis
  • Reaction center

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kimura, Y., Kawakami, T., Yu, L. J., Yoshimura, M., Kobayashi, M., & Wang-Otomo, Z. Y. (2015). Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. FEBS Letters, 589(15), 1761-1765. [37199]. https://doi.org/10.1016/j.febslet.2015.05.043