Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Yuuka Kimura, Tomoaki Kawakami, Long-Jiang Yu, Miku Yoshimura, Masayuki Kobayashi, Zheng Yu Wang-Otomo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

Original languageEnglish
Article number37199
Pages (from-to)1761-1765
Number of pages5
JournalFEBS Letters
Volume589
Issue number15
DOIs
Publication statusPublished - Jul 3 2015

Fingerprint

Chromatiaceae
Quinones
Sulfur
Vitamin K 2
Bacteria
Ubiquinone
Crystallization
Crystal structure
Membranes
Light

Keywords

  • Chromatophore
  • Electron transport
  • Light-harvesting
  • Photosynthesis
  • Reaction center

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

Cite this

Kimura, Y., Kawakami, T., Yu, L-J., Yoshimura, M., Kobayashi, M., & Wang-Otomo, Z. Y. (2015). Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. FEBS Letters, 589(15), 1761-1765. [37199]. https://doi.org/10.1016/j.febslet.2015.05.043

Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. / Kimura, Yuuka; Kawakami, Tomoaki; Yu, Long-Jiang; Yoshimura, Miku; Kobayashi, Masayuki; Wang-Otomo, Zheng Yu.

In: FEBS Letters, Vol. 589, No. 15, 37199, 03.07.2015, p. 1761-1765.

Research output: Contribution to journalArticle

Kimura, Y, Kawakami, T, Yu, L-J, Yoshimura, M, Kobayashi, M & Wang-Otomo, ZY 2015, 'Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum', FEBS Letters, vol. 589, no. 15, 37199, pp. 1761-1765. https://doi.org/10.1016/j.febslet.2015.05.043
Kimura Y, Kawakami T, Yu L-J, Yoshimura M, Kobayashi M, Wang-Otomo ZY. Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. FEBS Letters. 2015 Jul 3;589(15):1761-1765. 37199. https://doi.org/10.1016/j.febslet.2015.05.043
Kimura, Yuuka ; Kawakami, Tomoaki ; Yu, Long-Jiang ; Yoshimura, Miku ; Kobayashi, Masayuki ; Wang-Otomo, Zheng Yu. / Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. In: FEBS Letters. 2015 ; Vol. 589, No. 15. pp. 1761-1765.
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