Characterization of the oxidation reactions catalyzed by CYP2D enzyme in rat renal microsomes

Yasuhiro Masubuchi, Kyoko Yamamoto, Tokuji Suzuki, Toshiharu Horie, Shizuo Narimatsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Monooxygenase activities in rat renal microsomes were determined with the substrates of hepatic CYP2D enzymes. Seven kinds of CYP2D-mediated monooxygenase activities and immunochemically determined CYP2D contents in kidneys corresponded to approximately 3 % of those in livers. Debrisoquine 4- hydroxylase and bunitrolol 4-hydroxylase in renal microsomes were inhibited almost completely by the antibody against a CYP2D enzyme purified from rat liver. A marked strain difference (Wistar > Dark Agouti) in these activities was observed in kidney like in liver. The two hydroxylases were inhibited stereoselectively by quinine and quinidine both in renal and hepatic microsomes. Substrate stereoselectivity in (+)- and (-)-bunitrolol 4- hydroxylase activities in kidneys was also consistent with that in livers. These results suggested that the CYP2D enzyme(s) was expressed in the kidney at levels much less than in the liver but had similar functions to those in the liver.

Original languageEnglish
Pages (from-to)2431-2437
Number of pages7
JournalLife Sciences
Volume58
Issue number26
DOIs
Publication statusPublished - May 24 1996
Externally publishedYes

Fingerprint

Microsomes
Liver
Rats
Kidney
Oxidation
Enzymes
Mixed Function Oxygenases
Stereoselectivity
Cytochrome P-450 CYP2D6
Quinidine
Quinine
Substrates
Antibodies

Keywords

  • bunitrolol 4- hydroxylase
  • CYP2D
  • Dark Agouti rat
  • debrisoquine 4-hydroxylase
  • renal microsomes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Characterization of the oxidation reactions catalyzed by CYP2D enzyme in rat renal microsomes. / Masubuchi, Yasuhiro; Yamamoto, Kyoko; Suzuki, Tokuji; Horie, Toshiharu; Narimatsu, Shizuo.

In: Life Sciences, Vol. 58, No. 26, 24.05.1996, p. 2431-2437.

Research output: Contribution to journalArticle

Masubuchi, Y, Yamamoto, K, Suzuki, T, Horie, T & Narimatsu, S 1996, 'Characterization of the oxidation reactions catalyzed by CYP2D enzyme in rat renal microsomes', Life Sciences, vol. 58, no. 26, pp. 2431-2437. https://doi.org/10.1016/0024-3205(96)00247-0
Masubuchi, Yasuhiro ; Yamamoto, Kyoko ; Suzuki, Tokuji ; Horie, Toshiharu ; Narimatsu, Shizuo. / Characterization of the oxidation reactions catalyzed by CYP2D enzyme in rat renal microsomes. In: Life Sciences. 1996 ; Vol. 58, No. 26. pp. 2431-2437.
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