Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

Original languageEnglish
Pages (from-to)113-123
Number of pages11
JournalNew Phytologist
Volume195
Issue number1
DOIs
Publication statusPublished - Jul 2012

Fingerprint

transporters
Dissection
Zinc
zinc
promoter regions
Cadmium
uptake mechanisms
cadmium
shoots
Thlaspi
Xylem
Membrane Transport Proteins
Plant Cells
epidermis (plant)
Manganese
Systems Analysis
Plantae
Genetic Promoter Regions
Epidermis
xylem

Keywords

  • Cadmium (Cd) hyperaccumulation
  • Noccaea caerulescens
  • Zinc (Zn) hyperaccumulation
  • Zn uptake
  • ZNT1

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation. / Milner, Matthew J.; Craft, Eric; Yamaji, Naoki; Koyama, Emi; Ma, Jian Feng; Kochian, Leon V.

In: New Phytologist, Vol. 195, No. 1, 07.2012, p. 113-123.

Research output: Contribution to journalArticle

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