Characterization of the glycoproteins of bat-derived influenza viruses

Junki Maruyama, Naganori Nao, Hiroko Miyamoto, Ken Maeda, Hirohito Ogawa, Reiko Yoshida, Manabu Igarashi, Ayato Takada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recently found bat-derived influenza viruses (BatIVs) have hemagglutinin (HA) and neuraminidase (NA) gene segments distinct from those of previously known influenza A viruses. However, pathogenicities of these BatIVs remain unknown since infectious virus strains have not been isolated yet. To gain insight into the biological properties of BatIVs, we generated vesicular stomatitis viruses (VSVs) pseudotyped with the BatIV HA and NA. We found that VSVs pseudotyped with BatIV HAs and NAs efficiently infected particular bat cell lines but not those derived from primates, and that proteolytic cleavage with a trypsin-like protease was necessary for HA-mediated virus entry. Treatment of the susceptible bat cells with some enzymes and inhibitors revealed that BatIV HAs might recognize some cellular glycoproteins as receptors rather than the sialic acids used for the other known influenza viruses. These data provide fundamental information on the mechanisms underlying the cellular entry and host restriction of BatIVs.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalVirology
Volume488
DOIs
Publication statusPublished - Jan 15 2016
Externally publishedYes

Fingerprint

Orthomyxoviridae
Glycoproteins
Hemagglutinins
Vesicular Stomatitis
Neuraminidase
Viruses
Virus Internalization
Influenza A virus
Enzyme Inhibitors
Trypsin
Primates
Virulence
Peptide Hydrolases
Cell Line
Genes

Keywords

  • Bat influenza virus
  • Hemagglutinin
  • Pseudotyped vesicular stomatitis virus

ASJC Scopus subject areas

  • Virology

Cite this

Maruyama, J., Nao, N., Miyamoto, H., Maeda, K., Ogawa, H., Yoshida, R., ... Takada, A. (2016). Characterization of the glycoproteins of bat-derived influenza viruses. Virology, 488, 43-50. https://doi.org/10.1016/j.virol.2015.11.002

Characterization of the glycoproteins of bat-derived influenza viruses. / Maruyama, Junki; Nao, Naganori; Miyamoto, Hiroko; Maeda, Ken; Ogawa, Hirohito; Yoshida, Reiko; Igarashi, Manabu; Takada, Ayato.

In: Virology, Vol. 488, 15.01.2016, p. 43-50.

Research output: Contribution to journalArticle

Maruyama, J, Nao, N, Miyamoto, H, Maeda, K, Ogawa, H, Yoshida, R, Igarashi, M & Takada, A 2016, 'Characterization of the glycoproteins of bat-derived influenza viruses', Virology, vol. 488, pp. 43-50. https://doi.org/10.1016/j.virol.2015.11.002
Maruyama, Junki ; Nao, Naganori ; Miyamoto, Hiroko ; Maeda, Ken ; Ogawa, Hirohito ; Yoshida, Reiko ; Igarashi, Manabu ; Takada, Ayato. / Characterization of the glycoproteins of bat-derived influenza viruses. In: Virology. 2016 ; Vol. 488. pp. 43-50.
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