Characterization of the gene family for alternative oxidase from Arabidopsis thaliana

Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a, AOX1b, AOX1c and AOX2) of the AOX genes. Interestingly, two of the AOX genes (AOX1a and AOX1b) were located in tandem in a ca. 5 kb region on one of the chromosomes of Arabidopsis. Comparison between genomic and cDNA sequences of the four AOX genes showed that all AOX genes are divided by three introns and the positions of the introns in AOX1a, AOX1b, AOX1c and AOX2 are the same. We examined whether expression of Arabidopsis AOX genes, like the tobacco AOX1a gene, is enhanced by treatment with antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain. We found that, in young plants, the amount of Arabidopsis AOX1a mRNA was dramatically increased by addition of antimycin A, while the transcription of the other three genes (AOX1b, AOX1c and AOX2) did not respond to antimycin A. Amplification by RT-PCR showed that AOX1a and AOX1c were expressed in all organs examined (flowers and buds, stems, rosette, and roots of 8-week old plants). In contrast, transcripts of AOX1b were detected only in the flowers and buds, and transcripts of AOX2 were detected mainly in stems, rosette and roots. These results suggested that transcriptions of the four genes for alternative oxidase of Arabidopsis are differentially regulated.

Original languageEnglish
Pages (from-to)585-596
Number of pages12
JournalPlant Molecular Biology
Volume35
Issue number5
DOIs
Publication statusPublished - Nov 1997
Externally publishedYes

Fingerprint

Arabidopsis
Arabidopsis thaliana
Genes
Antimycin A
antimycin A
genes
Transcription
Introns
introns
Amplification
ubiquinol-cytochrome-c reductase
transcription (genetics)
buds
flowers
genomics
alternative oxidase
gene dosage
Polymerase Chain Reaction
stems
Gene Dosage

Keywords

  • Alternative oxidase
  • Arabidopsis thaliana
  • Cyanide-insensitive respiration
  • Differential expression
  • Gene family

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Characterization of the gene family for alternative oxidase from Arabidopsis thaliana. / Saisho, Daisuke; Nambara, Eiji; Naito, Satoshi; Tsutsumi, Nobuhiro; Hirai, Atsushi; Nakazono, Mikio.

In: Plant Molecular Biology, Vol. 35, No. 5, 11.1997, p. 585-596.

Research output: Contribution to journalArticle

Saisho, Daisuke ; Nambara, Eiji ; Naito, Satoshi ; Tsutsumi, Nobuhiro ; Hirai, Atsushi ; Nakazono, Mikio. / Characterization of the gene family for alternative oxidase from Arabidopsis thaliana. In: Plant Molecular Biology. 1997 ; Vol. 35, No. 5. pp. 585-596.
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