Characterization of the dCaMKII-GAL4 driver line whose expression is controlled by the Drosophila Ca2+/calmodulin-dependent protein kinase II promoter

Yoshiki Takamatsu, Hideki Nakagoshi, Mohammed Rachidi, Carmela Lopes, Yasuyoshi Nishida, Shunji Ohsako

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transgenic flies that can drive GAL4 expression under the control of the 7 kb 5′-region of the Drosophila Ca2+/calmodulin-dependent protein kinase II (dCaMKII) gene (dCaMKII-GAL4) were established. Characteristic features of this dCaMKII-GAL4 driven reporter expression were compatible with the endogenous dCaMKII expression pattern: The dCaMKII-GAL4 driven reporter gene was expressed preferentially in the central nervous system of the embryo and larvae. Reporter expression was also observed in the brain, thoracic ganglion, and gut of the adult. The whole-brain distribution and projections of dCaMKII-GAL4-expressing cells in the adults were visualized three-dimensionally by using UAS-linked reporter genes. Prominent signals of nuclear-localized β-Gal reporter gene expression were found in extensive brain regions, especially in the Kenyon cells of the mushroom body (MB), cells in the pars intercerebralis, and subesophageal ganglion (SOG). tau reporter gene expression highlighting neurite projections was detected in the MB lobes, median bundle, antennal lobe glomeruli, and fibers of clusters in the SOG, ventrolateral protocerebrum and superior lateral protocerebrum. These observations agree with those of a previous study mapping the dCaMKII-dependent memory circuits in courtship conditioning. Interestingly, green fluorescent protein reporter gene expression in adult MB lobes was predominantly observed in the α and β lobes with a core-deficient pattern, but not in the α′ and β′ lobes, similar to Fasciclin II immunoreactivity.

Original languageEnglish
Pages (from-to)237-252
Number of pages16
JournalCell and Tissue Research
Volume310
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Drosophila
Reporter Genes
Mushroom Bodies
Gene expression
Ganglia
Brain
Genes
Gene Expression
Courtship
Neurology
Neurites
Green Fluorescent Proteins
Diptera
Larva
Thorax
Embryonic Structures
Central Nervous System
Data storage equipment
Networks (circuits)

Keywords

  • CaMKII
  • Drosophila melanogaster (Insecta)
  • FasII
  • GAL4/UAS system
  • Mushroom body

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Characterization of the dCaMKII-GAL4 driver line whose expression is controlled by the Drosophila Ca2+/calmodulin-dependent protein kinase II promoter. / Takamatsu, Yoshiki; Nakagoshi, Hideki; Rachidi, Mohammed; Lopes, Carmela; Nishida, Yasuyoshi; Ohsako, Shunji.

In: Cell and Tissue Research, Vol. 310, No. 2, 2002, p. 237-252.

Research output: Contribution to journalArticle

Takamatsu, Yoshiki ; Nakagoshi, Hideki ; Rachidi, Mohammed ; Lopes, Carmela ; Nishida, Yasuyoshi ; Ohsako, Shunji. / Characterization of the dCaMKII-GAL4 driver line whose expression is controlled by the Drosophila Ca2+/calmodulin-dependent protein kinase II promoter. In: Cell and Tissue Research. 2002 ; Vol. 310, No. 2. pp. 237-252.
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