Abstract
Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B 12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10 9 and 10 12 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p <0.05) for the liver weight ratio (LWR) in a group fed with 10 12 cells per mouse when compared with the control group.
| Original language | English |
|---|---|
| Journal | Electronic Journal of Biotechnology |
| Volume | 13 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Fermented plant beverage
- Lactobacillus plantarum
- Phenyllactic acid
- Probiotics
- Rhodotorula mucilaginosa
- Weight control
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages. / Kantachote, Duangporn; Prachyakij, Pakorn; Charernjiratrakul, Wilawan; Ongsakul, Metta; Duangjitcharoen, Yodsawee; Chaiyasut, Chaiyavat; Nitoda, Teruhiko; Kanzaki, Hiroshi.
In: Electronic Journal of Biotechnology, Vol. 13, No. 5, 2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
AU - Kantachote, Duangporn
AU - Prachyakij, Pakorn
AU - Charernjiratrakul, Wilawan
AU - Ongsakul, Metta
AU - Duangjitcharoen, Yodsawee
AU - Chaiyasut, Chaiyavat
AU - Nitoda, Teruhiko
AU - Kanzaki, Hiroshi
PY - 2010
Y1 - 2010
N2 - Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B 12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10 9 and 10 12 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p <0.05) for the liver weight ratio (LWR) in a group fed with 10 12 cells per mouse when compared with the control group.
AB - Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B 12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10 9 and 10 12 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p <0.05) for the liver weight ratio (LWR) in a group fed with 10 12 cells per mouse when compared with the control group.
KW - Fermented plant beverage
KW - Lactobacillus plantarum
KW - Phenyllactic acid
KW - Probiotics
KW - Rhodotorula mucilaginosa
KW - Weight control
UR - http://www.scopus.com/inward/record.url?scp=79956278479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79956278479&partnerID=8YFLogxK
U2 - 10.2225/vol13-issue5-fulltext-1
DO - 10.2225/vol13-issue5-fulltext-1
M3 - Article
VL - 13
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
IS - 5
ER -