Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

Duangporn Kantachote, Pakorn Prachyakij, Wilawan Charernjiratrakul, Metta Ongsakul, Yodsawee Duangjitcharoen, Chaiyavat Chaiyasut, Teruhiko Nitoda, Hiroshi Kanzaki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B 12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10 9 and 10 12 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p <0.05) for the liver weight ratio (LWR) in a group fed with 10 12 cells per mouse when compared with the control group.

Original languageEnglish
JournalElectronic Journal of Biotechnology
Volume13
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Lactobacillus plantarum
Beverages
Probiotics
Growth
Yeasts
Acute Toxicity Tests
Rhodotorula
Lactobacillus casei
Weights and Measures
Inbred ICR Mouse
Control Groups
Acids
Vitamin B 12
Bacteremia
Bile Acids and Salts
Gas Chromatography-Mass Spectrometry
Health Status
Weight Gain
Spleen
Oxygen

Keywords

  • Fermented plant beverage
  • Lactobacillus plantarum
  • Phenyllactic acid
  • Probiotics
  • Rhodotorula mucilaginosa
  • Weight control

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages. / Kantachote, Duangporn; Prachyakij, Pakorn; Charernjiratrakul, Wilawan; Ongsakul, Metta; Duangjitcharoen, Yodsawee; Chaiyasut, Chaiyavat; Nitoda, Teruhiko; Kanzaki, Hiroshi.

In: Electronic Journal of Biotechnology, Vol. 13, No. 5, 2010.

Research output: Contribution to journalArticle

Kantachote, Duangporn ; Prachyakij, Pakorn ; Charernjiratrakul, Wilawan ; Ongsakul, Metta ; Duangjitcharoen, Yodsawee ; Chaiyasut, Chaiyavat ; Nitoda, Teruhiko ; Kanzaki, Hiroshi. / Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages. In: Electronic Journal of Biotechnology. 2010 ; Vol. 13, No. 5.
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