Abstract
We examined 14 patients with hepatitis C caused by infection with the hepatitis C virus-II genotype to understand differences in responsiveness to interferon. The patients were classified into two groups according to their response to interferon: eight responding and six non-responding patients. The 5′ noncoding and structural regions of the hepatitis C virus-II genome from each patient specimen were amplified by reverse transcription followed by the polymerase chain reaction. The nucleotide sequences of these amplified DNAs were then determined. By comparing the nucleotide sequences and the deduced amino acid sequences of samples from both groups, no group-specific sequence was observed in the analyzed regions despite the presence of considerable sequence diversity. However, additional cysteine residues were observed in half the responding group. The degree of micro-heterogeneity in hypervariable region 1 of the hepatitis C virus in relation to the sensitivity to interferon treatment was also examined; however, no significant correlation was observed. In addition, frequent alterations in the amino acid sequences were observed in hypervariable region 1 during the course of interferon treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 623-629 |
| Number of pages | 7 |
| Journal | Journal of Hepatology |
| Volume | 20 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1994 |
| Externally published | Yes |
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Keywords
- Envelope region
- Hepatitis C virus
- Hypervariable region
- Interferon treatment
- Sequence diversity
- Sequence variability
ASJC Scopus subject areas
- Gastroenterology
Cite this
Characterization of the 5′ noncoding and structural region of the hepatitis C virus genome from patients with non-A, non-B hepatitis responding differently to interferon treatment. / Nakazawa, Takahide; Kato, Nobuyuki; Ohkoshi, Showgo; Shibuya, Akitaka; Shimotohno, Kunitada.
In: Journal of Hepatology, Vol. 20, No. 5, 1994, p. 623-629.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the 5′ noncoding and structural region of the hepatitis C virus genome from patients with non-A, non-B hepatitis responding differently to interferon treatment
AU - Nakazawa, Takahide
AU - Kato, Nobuyuki
AU - Ohkoshi, Showgo
AU - Shibuya, Akitaka
AU - Shimotohno, Kunitada
PY - 1994
Y1 - 1994
N2 - We examined 14 patients with hepatitis C caused by infection with the hepatitis C virus-II genotype to understand differences in responsiveness to interferon. The patients were classified into two groups according to their response to interferon: eight responding and six non-responding patients. The 5′ noncoding and structural regions of the hepatitis C virus-II genome from each patient specimen were amplified by reverse transcription followed by the polymerase chain reaction. The nucleotide sequences of these amplified DNAs were then determined. By comparing the nucleotide sequences and the deduced amino acid sequences of samples from both groups, no group-specific sequence was observed in the analyzed regions despite the presence of considerable sequence diversity. However, additional cysteine residues were observed in half the responding group. The degree of micro-heterogeneity in hypervariable region 1 of the hepatitis C virus in relation to the sensitivity to interferon treatment was also examined; however, no significant correlation was observed. In addition, frequent alterations in the amino acid sequences were observed in hypervariable region 1 during the course of interferon treatment.
AB - We examined 14 patients with hepatitis C caused by infection with the hepatitis C virus-II genotype to understand differences in responsiveness to interferon. The patients were classified into two groups according to their response to interferon: eight responding and six non-responding patients. The 5′ noncoding and structural regions of the hepatitis C virus-II genome from each patient specimen were amplified by reverse transcription followed by the polymerase chain reaction. The nucleotide sequences of these amplified DNAs were then determined. By comparing the nucleotide sequences and the deduced amino acid sequences of samples from both groups, no group-specific sequence was observed in the analyzed regions despite the presence of considerable sequence diversity. However, additional cysteine residues were observed in half the responding group. The degree of micro-heterogeneity in hypervariable region 1 of the hepatitis C virus in relation to the sensitivity to interferon treatment was also examined; however, no significant correlation was observed. In addition, frequent alterations in the amino acid sequences were observed in hypervariable region 1 during the course of interferon treatment.
KW - Envelope region
KW - Hepatitis C virus
KW - Hypervariable region
KW - Interferon treatment
KW - Sequence diversity
KW - Sequence variability
UR - http://www.scopus.com/inward/record.url?scp=0028217214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028217214&partnerID=8YFLogxK
U2 - 10.1016/S0168-8278(05)80350-2
DO - 10.1016/S0168-8278(05)80350-2
M3 - Article
C2 - 7520922
AN - SCOPUS:0028217214
VL - 20
SP - 623
EP - 629
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 5
ER -