Characterization of surface properties of microbial transglutaminase using aqueous two-phase partitioning method

Hiroshi Umakoshi, Noriko Yoshimoto, Makoto Yoshimoto, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The surface properties of recombinant microbial transglutaminase (MTG) were characterized by varying the pH through the aqueous two-phase system (ATPS) method in order to select the effective refolding pathways. It was found that the MTG activity was lost and intrinsic tryptophan fluorescence was varied at pH values less than 3.8. In this pH region, the surface net charge and surface net hydrophobicity of the MTG, determined by the ATPS method, gradually increased. At around pH 3.5, the local hydrophobicity of the MTG was found to be maximal.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalSolvent Extraction Research and Development
Volume15
Publication statusPublished - 2008
Externally publishedYes

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Transglutaminases
Hydrophobicity
Surface properties
Fluorescence
Tryptophan

ASJC Scopus subject areas

  • Chemistry(all)
  • Filtration and Separation
  • Chemical Engineering(all)

Cite this

Characterization of surface properties of microbial transglutaminase using aqueous two-phase partitioning method. / Umakoshi, Hiroshi; Yoshimoto, Noriko; Yoshimoto, Makoto; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Solvent Extraction Research and Development, Vol. 15, 2008, p. 111-115.

Research output: Contribution to journalArticle

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