Characterization of substrate specificity of a rice silicon transporter, Lsi1

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    123 Citations (Scopus)

    Abstract

    Lsi1 (OsNIP2;1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl2 but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid; however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2;2 showed transport activity for silicic acid, whereas OsNIP1;1 and OsNIP3;1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore.

    Original languageEnglish
    Pages (from-to)679-686
    Number of pages8
    JournalPflugers Archiv European Journal of Physiology
    Volume456
    Issue number4
    DOIs
    Publication statusPublished - Jul 2008

    Keywords

    • Aquaporin
    • NIP subfamily
    • Phosphorylation
    • Rice
    • Silicon
    • Substrate specificity
    • ar/R selectivity filter

    ASJC Scopus subject areas

    • Physiology
    • Clinical Biochemistry
    • Physiology (medical)

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