Characterization of ripening-related genes involved in ethylene-independent low temperature-modulated ripening in ‘rainbow red’ kiwifruit during storage and on-vine

Oscar Witere Mitalo, William Olubero Asiche, Yuka Kasahara, Yasuaki Tosa, Willis Omondi Owino, Eric Gituma Mworia, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

‘Rainbow Red’ kiwifruit have been reported to gradually ripen during low temperature storage and on the vine in the absence of detectable ethylene. This study was conducted to compare the expression of ripening-related genes during storage at different temperatures and on the vine. Fruit at 5°C and 10°C ripened faster to eating quality within four weeks accompanied with increased expression of ripening-related genes: AcACO3, AcXET2, AcEXP1, AcPG, AcPMEi, AcSUS, AcβAMY1, AcβAMY2, AcGA2ox2, AcNAC3, AcNAC4, and AcMADS2. Fruit at 15°C required a longer period of eight weeks to attain eating quality in concurrence with delayed accumulation of the ripening-related genes. Fruit at 22°C ripened at the slowest rate and did not attain eating quality even after eight weeks, with very minimal accumulation of ripening-related genes. On-vine ripening occurred slowly at the early stages when the average field temperature was ~20°C, but the rate increased as the temperature dropped to ≤15°C accompanied by increased expression of ripening-related genes. These results indicate that both ripening on-vine and during low temperature storage are modulated by low temperature independent of ethylene.

Original languageEnglish
Pages (from-to)421-429
Number of pages9
JournalHorticulture Journal
Volume87
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

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kiwifruit
vines
ethylene
ripening
temperature
genes
ingestion
storage temperature
fruits

Keywords

  • 1-MCP
  • Field temperature
  • Gene expression
  • Ripening
  • Transcription factor

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Characterization of ripening-related genes involved in ethylene-independent low temperature-modulated ripening in ‘rainbow red’ kiwifruit during storage and on-vine. / Mitalo, Oscar Witere; Asiche, William Olubero; Kasahara, Yuka; Tosa, Yasuaki; Owino, Willis Omondi; Mworia, Eric Gituma; Ushijima, Koichiro; Nakano, Ryohei; Kubo, Yasutaka.

In: Horticulture Journal, Vol. 87, No. 3, 01.01.2018, p. 421-429.

Research output: Contribution to journalArticle

Mitalo, Oscar Witere ; Asiche, William Olubero ; Kasahara, Yuka ; Tosa, Yasuaki ; Owino, Willis Omondi ; Mworia, Eric Gituma ; Ushijima, Koichiro ; Nakano, Ryohei ; Kubo, Yasutaka. / Characterization of ripening-related genes involved in ethylene-independent low temperature-modulated ripening in ‘rainbow red’ kiwifruit during storage and on-vine. In: Horticulture Journal. 2018 ; Vol. 87, No. 3. pp. 421-429.
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